Krause D S, Theise N D, Collector M I, Henegariu O, Hwang S, Gardner R, Neutzel S, Sharkis S J
Department of Laboratory Medicine and, Yale University School of Medicine, New Haven, CT 06520, USA.
Cell. 2001 May 4;105(3):369-77. doi: 10.1016/s0092-8674(01)00328-2.
Purification of rare hematopoietic stem cell(s) (HSC) to homogeneity is required to study their self-renewal, differentiation, phenotype, and homing. Long-term repopulation (LTR) of irradiated hosts and serial transplantation to secondary hosts represent the gold standard for demonstrating self-renewal and differentiation, the defining properties of HSC. We show that rare cells that home to bone marrow can LTR primary and secondary recipients. During the homing, CD34 and SCA-1 expression increases uniquely on cells that home to marrow. These adult bone marrow cells have tremendous differentiative capacity as they can also differentiate into epithelial cells of the liver, lung, GI tract, and skin. This finding may contribute to clinical treatment of genetic disease or tissue repair.
为了研究稀有造血干细胞（HSC）的自我更新、分化、表型和归巢，需要将其纯化至同质状态。对受辐照宿主进行长期重建造血（LTR）以及向二级宿主进行连续移植，是证明自我更新和分化（HSC的决定性特性）的金标准。我们发现，归巢至骨髓的稀有细胞能够使一级和二级受体实现长期重建造血。在归巢过程中，归巢至骨髓的细胞上CD34和SCA-1的表达会独特地增加。这些成年骨髓细胞具有巨大的分化能力，因为它们还能分化为肝脏、肺、胃肠道和皮肤的上皮细胞。这一发现可能有助于遗传病的临床治疗或组织修复。
