Cane E M, Villee C A
Prostaglandins. 1975 Feb;9(2):281-8. doi: 10.1016/0090-6980(75)90032-5.
Slices of human endometrium obtained from hysterectomy specimens were cultured for 48 hours in an organ culture medium supplemented with ethanol (control, vehicle), 17-beta-estradiol (.5 mug/ml), or progesterone (.5 mug/ml). Uncultured endometrial tissue, cultured tissues, and the media were assayed for prostaglandin F (PGF) by a radioimmunoassay technique. Hematoxylin and eosin and periodic acid-Schiff stain histologic controls were done on all tissues. The concentrations of PGF in picograms/milligram, corrected for percent recovery, in the differently treated tissues were: preculture 298; culture control 2210; estrogen-treated 2680; progesterone-treated 1260. All differences except those between estrogen and control (p greater than .10) and progesterone and control (p less than .10) are significant at the p = .02 level or better. Progesterone appears to inhibit PGF synthesis which occurs during in vitro culture of human endometrium; estrogen tended to increase PGF synthesis in this system.
从子宫切除标本中获取的人子宫内膜切片在补充了乙醇（对照，赋形剂）、17-β-雌二醇（0.5微克/毫升）或孕酮（0.5微克/毫升）的器官培养基中培养48小时。通过放射免疫测定技术对未培养的子宫内膜组织、培养的组织和培养基进行前列腺素F（PGF）检测。对所有组织进行苏木精和伊红染色以及过碘酸希夫染色的组织学对照。经不同处理的组织中，经回收率校正后，PGF的浓度（皮克/毫克）分别为：培养前298；培养对照2210；雌激素处理组2680；孕酮处理组1260。除雌激素与对照组之间（p＞0.10）以及孕酮与对照组之间（p＜0.10）的差异外，所有差异在p = 0.02水平或更低水平上均具有显著性。孕酮似乎抑制人子宫内膜体外培养期间发生的PGF合成；在该系统中，雌激素倾向于增加PGF合成。
