Jeon Y K, Kim S Y, Jeon C J
Department of Biology, College of Natural Sciences, Kyungpook National University, Taegu, Korea.
Mol Cells. 2001 Apr 30;11(2):250-6.
The morphology of calretinin- and tyrosine hydroxylase-immunoreactive (IR) neurons in adult pig retina was studied. These neurons were identified using antibody immunocytochemistry. Calretinin immunoreactivity was found in numerous cell bodies in the ganglion cell layer. Large ganglion cells, however, were not labeled. In the inner nuclear layer, the regular distribution of calretinin-IR neurons, the inner marginal location of their cell bodies in the inner nuclear layer, and the distinctive bilaminar morphologies of their dendritic arbors in the inner plexiform layer suggested that these calretinin-IR cells were AII amacrine cells. Calretinin immunoreactivity was observed in both A-and B-type horizontal cells. Neurons in the photoreceptor cell layer were not labeled by this antibody. The great majority of tyrosine hydroxylase-IR neurons were located at the innermost border of the inner nuclear layer (conventional amacrines). The processes were monostratified and ran laterally within layer 1 of the inner plexiform layer. Some of the tyrosine hydroxylase-IR neurons were located in the ganglion cell layer (displaced amacrines). The processes of displaced tyrosine hydroxylase-IR amacrine cells were also located within layer 1 of the inner plexiform layer. Some processes of a few neurons were located in the outer plexiform layer. A very low density of neurons had additional bands of tyrosine hydroxylase-IR processes in the middle and deep layers of the inner plexiform layer. The processes of tyrosine hydroxylase-IR neurons extended radially over a wide area and formed large, moderately branched dendritic fields. These processes occasionally had varicosities and formed "dendritic rings". These results indicate that calretinin- and tyrosine hydroxylase-IR neurons represent specific neuronal cell types in the pig retina.
研究了成年猪视网膜中钙视网膜蛋白和酪氨酸羟化酶免疫反应性（IR）神经元的形态。使用抗体免疫细胞化学鉴定这些神经元。在神经节细胞层的许多细胞体中发现了钙视网膜蛋白免疫反应性。然而，大型神经节细胞未被标记。在内核层中，钙视网膜蛋白免疫反应性神经元的规则分布、其细胞体在内核层的内边缘位置以及它们在内网状层中独特的双极形态表明这些钙视网膜蛋白免疫反应性细胞是AII无长突细胞。在A型和B型水平细胞中均观察到钙视网膜蛋白免疫反应性。光感受器细胞层中的神经元未被该抗体标记。绝大多数酪氨酸羟化酶免疫反应性神经元位于内核层的最内边界（传统无长突细胞）。其突起单分层并在内网状层的第1层内横向延伸。一些酪氨酸羟化酶免疫反应性神经元位于神经节细胞层（移位无长突细胞）。移位的酪氨酸羟化酶免疫反应性无长突细胞的突起也位于内网状层的第1层内。少数神经元的一些突起位于外网状层。在内网状层的中层和深层中，非常低密度的神经元具有额外的酪氨酸羟化酶免疫反应性突起带。酪氨酸羟化酶免疫反应性神经元的突起径向延伸到广泛区域并形成大的、适度分支的树突野。这些突起偶尔有膨体并形成“树突环”。这些结果表明钙视网膜蛋白和酪氨酸羟化酶免疫反应性神经元代表猪视网膜中的特定神经元细胞类型。
