Linser P, Bruning H, Armentrout R W
J Virol. 1979 Aug;31(2):537-45. doi: 10.1128/JVI.31.2.537-545.1979.
The uptake of minute virus of mice into cells in tissue culture was examined biochemically and by electron microscopy. Cell-virus complexes were formed at 4 degrees C, and uptake of virus was followed after the cells were shifted to 37 degrees C. The infectious particles appeared to enter cells at 37 degrees C by a two-step process. The first and rapid phase was measured by the resistance of cell-bound virus to elution by EDTA. The bulk of the bound virus particles became refractory to elution with EDTA within 30 min of incubation at 37 degrees C. The infectious particles became resistant to EDTA elution at the same rate. The second, slower phase of the uptake process was measured by the resistance of infectious particles to neutralization by antiserum. This process was complete within 2 h of incubation at 37 degrees C. During this 2-h period, labeled viral DNA became progressively associated with the nuclear fraction of disrupted cells. The uptake of infectious virus could occur during the G1 phase of the cell cycle and was not an S phase-specific event. The uptake process was not the cause of the S phase dependence of minute virus of mice replication. In electron micrographs, virus absorbed to any area of the cell surface appeared to be taken into the cell by pinocytosis.
通过生物化学方法和电子显微镜对小鼠微小病毒在组织培养细胞中的摄取情况进行了研究。细胞 - 病毒复合物在4℃形成，将细胞转移至37℃后追踪病毒摄取情况。感染性颗粒似乎在37℃通过两步过程进入细胞。第一步快速阶段通过细胞结合病毒对EDTA洗脱的抗性来衡量。在37℃孵育30分钟内，大部分结合的病毒颗粒对EDTA洗脱变得具有抗性。感染性颗粒以相同速率对EDTA洗脱产生抗性。摄取过程的第二步较慢阶段通过感染性颗粒对抗血清中和的抗性来衡量。此过程在37℃孵育2小时内完成。在这2小时期间，标记的病毒DNA逐渐与破碎细胞的核部分相关联。感染性病毒的摄取可发生在细胞周期的G1期，并非S期特异性事件。摄取过程不是小鼠微小病毒复制对S期依赖性的原因。在电子显微镜照片中，吸附在细胞表面任何区域的病毒似乎通过胞饮作用进入细胞。
