Linser P, Bruning H, Armentrout R W
J Virol. 1977 Oct;24(1):211-21. doi: 10.1128/JVI.24.1.211-221.1977.
The early interactions between parvoviruses and host cells have not been extensively described previously. In this study we have characterized some aspects of viral binding to the cell surface and demonstrated the existence of specific cellular receptor sites for minute virus of mice (MVM) on two murine cell lines that are permissive for viral growth. The interaction had a pH optimum of 7.0 to 7.2, and both the rate and extent of the reactions were slightly affected by temperature. Mouse A-9 cells (L-cell derivative) had approximately 5 X 10(5) specific MVM binding sites per cell, and Friend erythroleukemia cells had 1.5 X 10(5) MVM sites per cell. In contrast, the nonpermissive mouse lymphoid cell line L1210 lacked specific viral receptors. Also, cloned lines of A-9 cells resistant to viral infection have been isolated. One of these lines lacked the "specific" virus attachment sites but exhibited low levels of nonsaturable virus binding. Based on these examples, infectivity is correlated with the presence of specific viral receptors on the cell surface.
细小病毒与宿主细胞之间的早期相互作用此前尚未得到广泛描述。在本研究中，我们对病毒与细胞表面结合的某些方面进行了表征，并证明在两种允许病毒生长的小鼠细胞系上存在小鼠微小病毒（MVM）的特异性细胞受体位点。这种相互作用的最适pH为7.0至7.2，反应速率和程度均受温度轻微影响。小鼠A-9细胞（L细胞衍生物）每个细胞约有5×10⁵个特异性MVM结合位点，Friend红白血病细胞每个细胞有1.5×10⁵个MVM位点。相比之下，非允许性小鼠淋巴细胞系L1210缺乏特异性病毒受体。此外，已分离出对病毒感染具有抗性的A-9细胞克隆系。其中一个克隆系缺乏“特异性”病毒附着位点，但表现出低水平的非饱和病毒结合。基于这些实例，感染性与细胞表面特异性病毒受体的存在相关。
