Shukla V B., Parasu Veera U, Kulkarni P R., Pandit A B.
Department of Chemical Technology, Food and Fermentation Technology Division, University of Mumbai (UDCT), Matunga, 400 019, Mumbai, India
Biochem Eng J. 2001 Jul;8(1):19-29. doi: 10.1016/s1369-703x(00)00130-3.
The gas-liquid mass transfer coefficient K(L)a in the fermenter is a strong function of mode of energy dissipation and physico-chemical properties of the liquid media. A combination of disc turbine (DT) and pitched blade turbine down flow (PTD) impellers has been tested in laboratory bioreactor for gas hold-up and gas-liquid mass transfer performance for the growth and biotransformation medium for an yeast isolate VS1 capable of biotransforming benzaldehyde to L-phenyl acetyl carbinol (L-PAC) and compared with those in water.Correlations have been developed for the prediction of the fractional gas hold-up and gas-liquid mass transfer coefficient for the above media. The mass transfer coefficient and respiration rate have been determined in the shake flask for the growth as well as for biotransformation medium. These results, then have been used to optimize the operating parameters (impeller speed and aeration) for growth and biotransformation in a laboratory bioreactor. The comparison of cell mass production and L-PAC production in the bioreactor has been done with that obtained in shake flask studies.
发酵罐中的气液传质系数K(L)a是能量耗散方式和液体介质物理化学性质的强函数。在实验室生物反应器中，对圆盘涡轮（DT）和斜叶涡轮下流式（PTD）叶轮的组合进行了测试，以研究其对一种能够将苯甲醛生物转化为L-苯基乙酰甲醇（L-PAC）的酵母分离株VS1的生长和生物转化培养基的持气率和气液传质性能，并与在水中的情况进行了比较。已建立了用于预测上述培养基的气体持留分数和气液传质系数的关联式。在摇瓶中测定了生长培养基和生物转化培养基的传质系数和呼吸速率。然后，利用这些结果优化了实验室生物反应器中生长和生物转化的操作参数（叶轮转速和通气量）。已将生物反应器中细胞质量产量和L-PAC产量与摇瓶研究中获得的结果进行了比较。
