酵母中的呼吸链复合体I：从专性需氧酵母解脂耶氏酵母中分离出编码NADH：泛醌氧化还原酶核苷酸结合亚基的基因NUO51。

The respiratory complex I in yeast: isolation of a gene NUO51 coding for the nucleotide-binding subunit of NADH:ubiquinone oxidoreductase from the obligately aerobic yeast Yarrowia lipolytica.

作者信息

Rycovská A, Szabo R, Tomáska L, Nosek J

机构信息

Department of Biochemistry, Faculty of Science, Comenius University, 842 15 Bratislava, Slovakia.

出版信息

Folia Microbiol (Praha). 2000;45(5):429-33. doi: 10.1007/BF02817616.

DOI:10.1007/BF02817616

PMID:11357863

Abstract

We have isolated a gene NUO51 coding for a homologue of the nucleotide-binding subunit of mitochondrial respiratory chain linked NADH:ubiquinone oxidoreductase from the obligately aerobic yeast Yarrowia lipolytica. DNA sequencing revealed a 1464 bp open reading frame encoding a protein with predicted molar mass of about 53.7 kDa. The sequence is highly conserved with its counterparts from filamentous fungi and represents the first yeast homologue of the NADH-binding subunit (51 kDa) of the respiratory complex 1. In addition, PFGE and Southern hybridization analysis indicate that NUO51 is a single copy gene in the genome of Y. lipolytica. The expression of NUO51 by Northern blot analysis was also examined.

摘要

我们从专性需氧酵母解脂耶氏酵母中分离出了一个基因NUO51，该基因编码线粒体呼吸链连接的NADH：泛醌氧化还原酶核苷酸结合亚基的同源物。DNA测序揭示了一个1464 bp的开放阅读框，编码一种预测摩尔质量约为53.7 kDa的蛋白质。该序列与其在丝状真菌中的对应序列高度保守，代表了呼吸复合体1的NADH结合亚基（51 kDa）的首个酵母同源物。此外，脉冲场凝胶电泳（PFGE）和Southern杂交分析表明，NUO51是解脂耶氏酵母基因组中的单拷贝基因。我们还通过Northern印迹分析检测了NUO51的表达。