酿酒酵母线粒体基因组中ND基因的分布及膜醭毕赤酵母的线粒体DNA结构。

The distribution of ND genes in yeast mitochondrial genomes and the mitochondrial DNA structure of Pichia membranaefacens.

作者信息

Kitano H, Sekito T, Ishitomi H, Yoshida K

机构信息

Department of Biological Science, Faculty of Science, Hiroshima University, Japan.

出版信息

Nucleic Acids Symp Ser. 1995(34):23-4.

PMID:8841533

Abstract

For long time, it has been believed that the yeast mitochondrial (mt) genome lacks NADH dehydrogenase subunit genes which are designated ND genes. However, our complete mtDNA sequencing of yeast Hansenula wingei led us to the first finding of seven mitochondrial ND genes. We investigated the distribution of ND genes in mtDNAs of other yeasts including Pichia membranaefaciens, Yarrowia lypolitica, Candida maltosa, Saccharomyces kluyveri and Saccharomyces exiguus. By Southern hybridization with probes of H. wingei's ND1, 2 and 5 genes, we detected positive signals on mtDNAs in P. membranaefaciens, Y. lypolitica, and C. maltosa. To confirm this, we cloned and sequenced DNA fragment of ND5 gene in P. membranaefaciens. We have discussed the sequence homology and genome structure.

摘要

长期以来，人们一直认为酵母线粒体（mt）基因组缺乏被称为ND基因的NADH脱氢酶亚基基因。然而，我们对汉逊酵母完整的线粒体DNA测序首次发现了七个线粒体ND基因。我们研究了ND基因在其他酵母线粒体DNA中的分布，这些酵母包括膜醭毕赤酵母、解脂耶氏酵母、麦芽糖假丝酵母、克鲁维酵母和微小酵母。通过用汉逊酵母的ND1、2和5基因探针进行Southern杂交，我们在膜醭毕赤酵母、解脂耶氏酵母和麦芽糖假丝酵母的线粒体DNA上检测到了阳性信号。为了证实这一点，我们克隆并测序了膜醭毕赤酵母中ND5基因的DNA片段。我们还讨论了序列同源性和基因组结构。