Williamson V M, Doi R H
Mol Gen Genet. 1979 Jul 2;174(1):47-52. doi: 10.1007/BF00433304.
The relationship between sigma (sigma) and delta (delta) factors of Bacillus subtilis RNA polymerase has been analyzed during initiation of RNA synthesis. When core enzyme (E) containing delta factor (E delta) binds to DNA, the delta factor is released with the formation of an E-DNA complex. The addition of sigma to the E-DNA complex results in the formation of a stable E sigma-DNA complex which can synthesize RNA upon addition of nucleoside triphosphates. Sigma factor, significantly, is not released from the core during RNA synthesis. These results suggest that delta and sigma factors can act sequentially during initiation of RNA synthesis with delta acting as a DNA recognition factor and sigma acting as an initiation factor. The results do not preclude the possibility that E sigma can initiate RNA synthesis correctly since E sigma alone can bind to DNA and initiate RNA synthesis.
在RNA合成起始过程中，对枯草芽孢杆菌RNA聚合酶的σ（西格玛）因子和δ（德尔塔）因子之间的关系进行了分析。当含有δ因子（Eδ）的核心酶（E）与DNA结合时，δ因子会随着E-DNA复合物的形成而释放。向E-DNA复合物中添加σ因子会导致形成稳定的Eσ-DNA复合物，该复合物在添加核苷三磷酸后能够合成RNA。值得注意的是，在RNA合成过程中，σ因子不会从核心酶上释放。这些结果表明，δ因子和σ因子在RNA合成起始过程中可以依次发挥作用，其中δ因子作为DNA识别因子，而σ因子作为起始因子。这些结果并不排除Eσ能够正确起始RNA合成的可能性，因为单独的Eσ就能与DNA结合并起始RNA合成。
