Carter H L, Moran C P
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9438-42. doi: 10.1073/pnas.83.24.9438.
In Bacillus subtilis transcription of spoVG is activated within minutes after the initiation of sporulation. Mutations in several spo0 genes prevent the activation of spoVG transcription. We have found a sigma-like protein that is capable of directing core RNA polymerase to use the spoVG promoter in an in vitro run-off transcription assay. This sigma-like protein was not found to be associated with RNA polymerase in a spo0A or spo0B mutant but was present in a spo0H mutant. We suggest that one role of the spo0A gene product in transcription of spoVG is the modulation of RNA polymerase activity by this sigma-like protein.
在枯草芽孢杆菌中，spoVG的转录在芽孢形成开始后的几分钟内被激活。几个spo0基因的突变会阻止spoVG转录的激活。我们发现了一种类σ因子蛋白，在体外延伸转录试验中，它能够引导核心RNA聚合酶使用spoVG启动子。在spo0A或spo0B突变体中未发现这种类σ因子蛋白与RNA聚合酶相关联，但在spo0H突变体中存在。我们认为spo0A基因产物在spoVG转录中的一个作用是通过这种类σ因子蛋白调节RNA聚合酶的活性。
