同位素示踪结合三氯乙酸沉淀法测定小鼠血浆中神经生长因子浓度
[Isotope tracer combined with trichloroacetic acid precipitation method for determination of nerve growth factor concentration in mouse plasma].
作者信息
Tang G, Jiang G, Zhang Y, Chen L, Wang S
机构信息
PUMC Hospital, CAMS and PUMC, Beijing 100730.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1998 Jun;20(3):203-6.
OBJECTIVE
To establish the radioisotope (125I) tracer method combined with trichloroacetic acid (TCA) precipitation for the determination of nerve growth factor (NGF) in mouse plasma.
METHODS
Radioisotope tracer labelling method combined with TCA precipitation was used to determine NGF in mouse plasma.
RESULTS
The detection limit of the method for NGF in plasma was 2.0 ng/ml. The intra-assay cv (CV) and the inter-assay CV were all less than 10%. The assay linearity was determined over the range of 12.5-400 ng/ml in plasma and the correlation coefficient was 0.9990. The average recovery of the method for NGF was 95.21%.
CONCLUSIONS
The radioisotope tracer method combined with TCA precipitation is stable, sensitive and reliable. It is suitable for the determination of NGF concentration in mouse plasma.
目的
建立放射性同位素（125I）示踪法结合三氯乙酸（TCA）沉淀法测定小鼠血浆中神经生长因子（NGF）的含量。
方法
采用放射性同位素示踪标记法结合TCA沉淀法测定小鼠血浆中的NGF。
结果
该方法对血浆中NGF的检测限为2.0 ng/ml。批内变异系数（CV）和批间CV均小于10%。在血浆12.5 - 400 ng/ml范围内测定了该方法的线性，相关系数为0.9990。该方法对NGF的平均回收率为95.21%。
结论
放射性同位素示踪法结合TCA沉淀法稳定、灵敏、可靠。适用于测定小鼠血浆中NGF的浓度。
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