Brugmans F, Venner M, Menzel D, Mischke R
Klinik für Pferde, Tierärztliche Hochschule Hannover.
Dtsch Tierarztl Wochenschr. 1998 Feb;105(2):58-61.
The purpose of this study was to investigate the usefulness of two heat-precipitation techniques (Schalm- and Millar-method) as screening tests to measure plasma fibrinogen concentration in horses. Based on the measurement of samples from 108 different horses, the coefficient of correlation (CC) for the relationship between the results with the Schalm- and with the reference-method (Jacobsson) were much lower (r = 0.78) than between the Millar- and Jacobsson-method (r = 0.94). Furthermore the Schalm-method was less precise as reflected by the greater coefficient of variation (CV, within-run precision) with a sample of low-limit fibrinogen concentration (CV = 47.4 %) and with a sample of high fibrinogen concentration (CV = 35.6 %) than the Millar-method (CV = 11.1 resp. 2.9 %). In 40 healthy horses, aged 3 to 19 years, fibrinogen values ranged from 1.82 to 4.94 g/l (2.5-97.5 %-quantil). The Millar-Method is recommended as a simple and suitable heat-precipitation assay for fibrinogen determination in the horse.
本研究的目的是调查两种热沉淀技术（Schalm法和Millar法）作为筛选试验来测定马血浆纤维蛋白原浓度的实用性。基于对108匹不同马匹样本的测量，Schalm法结果与参考方法（Jacobsson法）之间关系的相关系数（CC）远低于Millar法与Jacobsson法之间的相关系数（r = 0.78 vs r = 0.94）。此外，Schalm法的精密度较低，低纤维蛋白原浓度样本（CV = 47.4%）和高纤维蛋白原浓度样本（CV = 35.6%）的变异系数（CV，批内精密度）均高于Millar法（分别为CV = 11.1%和2.9%）。在40匹3至19岁的健康马匹中，纤维蛋白原值范围为1.82至4.94 g/l（2.5 - 97.5%分位数）。Millar法被推荐为一种简单且合适的热沉淀法，用于测定马的纤维蛋白原。
