Ishikawa Y, Ito K, Akasaka Y, Ishii T, Masuda T, Zhang L, Akishima Y, Kiguchi H, Nakajima K, Hata Y
Department of Pathology, Toho University School of Medicine, 5-21-16 Ohmori-nishi, Ohta-ku, Tokyo 143-8540, Japan.
Atherosclerosis. 2001 May;156(1):29-37. doi: 10.1016/s0021-9150(00)00610-9.
Cholesteryl ester transfer protein (CETP) has been considered to mediate the transfer of cholesteryl ester from arterial wall, however, the distribution and production of CETP in human arterial wall remains unclear. Present study histopathologically demonstrated the distribution of CETP and CETP mRNA in the human aortic wall by immunohistochemistry and in situ hybridization. While CETP was constantly distributed in the media, the protein was recognized within the intima with fibrocellular thickening and atherosclerotic intima. Double immunostaining methods demonstrated CETP expression in smooth muscle cells in the intima and media. CETP mRNA was detected not only in intimal cells but medial smooth muscle cells. Intimal cells expressing CETP mRNA were considered to be monocyte-derived macrophages and smooth muscle cells by immunohistochemistries using two antibodies against smooth muscle actin and human macrophage on the subserial sections. Our in vivo study provides that CETP is produced by smooth muscle cells in the intima and media of human aorta, and it is suggested that arterial smooth muscle cells positively participate in the removal of excessive cholesteryl ester from the arterial wall by CETP production.
胆固醇酯转运蛋白（CETP）被认为可介导胆固醇酯从动脉壁的转运，然而，CETP在人动脉壁中的分布及产生情况仍不清楚。本研究通过免疫组织化学和原位杂交从组织病理学角度证实了CETP和CETP mRNA在人主动脉壁中的分布。虽然CETP持续分布于中膜，但在内膜有纤维细胞增厚和动脉粥样硬化内膜处可检测到该蛋白。双重免疫染色方法证实内膜和平滑肌细胞中存在CETP表达。不仅在内膜细胞中检测到CETP mRNA，中膜平滑肌细胞中也有。通过对连续切片使用两种分别针对平滑肌肌动蛋白和人巨噬细胞的抗体进行免疫组织化学分析，发现表达CETP mRNA的内膜细胞被认为是单核细胞衍生的巨噬细胞和平滑肌细胞。我们的体内研究表明，CETP由人主动脉内膜和平滑肌细胞产生，提示动脉平滑肌细胞通过产生CETP积极参与从动脉壁清除过量胆固醇酯的过程。
