Vinagre C, Martínez M J, Vogel M, Traipe L, Stoppel J, Squella O, Srur M, Charlín R
Departamento de Oftalmología, Facultad de Medicina, Universidad de Chile.
Rev Med Chil. 2001 Mar;129(3):259-63.
Herpes simplex virus (HSV) infection of the cornea is a leading cause of blindness in occidental countries and a common recurrent manifestation of it is the immune stromal keratitis (ISK). However, it is not known whether active viral replication occurs during the acute phase of the disease, because isolation of the virus by conventional culture techniques has not been accomplished.
To establish the presence of HSV in patients with ISK.
Fourteen corneal swabbing samples, from active diseased eyes of patients with clinical diagnosis of ISK, were submitted to Herpchek and PCR for the identification of HSV antigens and genome.
All ISK samples were negative by both techniques.
It was not possible to identify HSV antigens nor their genome by the methodology used. It is likely that, they can't be detected in corneal superficial layers or probably there is no viral replication at this stage of the disease, so antiviral therapy should be reconsidered.
单纯疱疹病毒（HSV）感染角膜是西方国家失明的主要原因，其常见的复发表现是免疫性基质性角膜炎（ISK）。然而，目前尚不清楚在疾病急性期是否发生病毒的活跃复制，因为通过传统培养技术分离病毒尚未成功。
确定免疫性基质性角膜炎（ISK）患者中HSV的存在情况。
从临床诊断为ISK的患者的活动性患眼中采集了14份角膜拭子样本，进行疱疹病毒检测（Herpchek）和聚合酶链反应（PCR）以鉴定HSV抗原和基因组。
两种技术检测的所有免疫性基质性角膜炎（ISK）样本均为阴性。
采用的方法无法鉴定HSV抗原及其基因组。很可能在角膜表层无法检测到它们，或者在疾病的这个阶段可能没有病毒复制，因此应重新考虑抗病毒治疗。
