Komaki G, Matsumoto Y, Nishikata H, Kawai K, Nozaki T, Takii M, Sogawa H, Kubo C
Division of Psychosomatic Research, National Institute of Mental Health, National Center of Neurology and Psychiatry, Ichikawa, Japan.
Eur J Endocrinol. 2001 Jun;144(6):645-51. doi: 10.1530/eje.0.1440645.
Leptin, neuropeptide-Y (NPY) and orexin are peptides regulating energy metabolism and appetite control. NPY and orexin are mainly found in the central nervous system and they have also recently been found in the peripheral nervous system. We investigated how fasting affects changes in circulating concentrations of these peptides and their association with nutritional and metabolic parameters in humans.
Ten non-obese female patients with psychosomatic disorders fasted for 7 or 10 days. Blood samples were collected at 0800 h before fasting, on the 3rd and 7th days during the fast (with an additional sample taken on the 10th day when the fasting continued for 10 days) and on the 3rd and 7th days of refeeding. We measured blood concentrations of orexin-A, NPY, leptin, adrenocorticotropin, cortisol, insulin, C-peptide, glucose, and beta-hydroxybutyrate.
Body mass index and plasma leptin concentrations concomitantly and significantly decreased during fasting, whereas serum orexin-A concentrations significantly increased and were negatively correlated with plasma leptin concentrations. Plasma NPY concentrations decreased slightly but were not significantly different from the prefasting values, and no significant relationship with leptin or orexin-A was found. Orexin-A and leptin concentrations showed a significant inverse correlation with serum glucose, insulin, C-peptide, and beta-hydroxybutyrate concentrations. Only changes in plasma leptin concentrations showed a significant negative correlation with serum cortisol concentrations. All the measured indices which changed during fasting returned to the prefasting concentrations by the 7th day of refeeding.
Peripheral orexin-A and leptin concentrations inversely change during fasting, which is significantly correlated with energy metabolism in humans.
瘦素、神经肽Y（NPY）和食欲素是调节能量代谢和食欲控制的肽类。NPY和食欲素主要存在于中枢神经系统，最近也在周围神经系统中被发现。我们研究了禁食如何影响这些肽类循环浓度的变化及其与人类营养和代谢参数的关联。
10名患有心身疾病的非肥胖女性患者禁食7天或10天。在禁食前的08:00采集血样，在禁食期间的第3天和第7天（禁食持续10天时在第10天额外采集一份样本）以及再进食的第3天和第7天采集血样。我们测量了食欲素-A、NPY、瘦素、促肾上腺皮质激素、皮质醇、胰岛素、C肽、葡萄糖和β-羟基丁酸的血浓度。
禁食期间体重指数和血浆瘦素浓度同时显著下降，而血清食欲素-A浓度显著升高且与血浆瘦素浓度呈负相关。血浆NPY浓度略有下降，但与禁食前的值无显著差异，且未发现与瘦素或食欲素-A有显著关系。食欲素-A和瘦素浓度与血清葡萄糖、胰岛素、C肽和β-羟基丁酸浓度呈显著负相关。只有血浆瘦素浓度的变化与血清皮质醇浓度呈显著负相关。禁食期间所有变化的测量指标在再进食第7天时恢复到禁食前浓度。
禁食期间外周食欲素-A和瘦素浓度呈反向变化，这与人类能量代谢显著相关。
