通过核磁共振确定细胞穿透肽穿膜肽在十二烷基硫酸钠胶束中的位置。

The position of the cell penetrating peptide penetratin in SDS micelles determined by NMR.

作者信息

Lindberg M, Gräslund A

机构信息

Department of Biochemistry and Biophysics, The Arrhenius Laboratories, Stockholm University, S-106 91, Stockholm, Sweden.

出版信息

FEBS Lett. 2001 May 18;497(1):39-44. doi: 10.1016/s0014-5793(01)02433-4.

DOI:10.1016/s0014-5793(01)02433-4

PMID:11376659

Abstract

Penetratin is a 16 residue peptide, RQI KIWFQ NRRMK WKK-amide, with the ability to penetrate cell membranes and a sequence taken from the homeodomain of the Drosophila Antennapedia transcription factor. 600 MHz 1H-nuclear magnetic resonance has been used to study the structure and location of penetratin interacting with a sodium dodecyl sulphate micelle. The positioning of penetratin in the micelle was studied by adding paramagnetic probes (Mn2+ ions, 5-doxyl and 12-doxyl stearic acid) to the solvent. The results show that the peptide is a straight helix positioned with its C-terminus deep inside the micelle and its N-terminus near the surface of the micelle.

摘要

穿膜肽是一种由16个氨基酸残基组成的肽，序列为RQI KIWFQ NRRMK WKK-酰胺，具有穿透细胞膜的能力，其序列取自果蝇触角足转录因子的同源结构域。已利用600兆赫的氢核磁共振研究穿膜肽与十二烷基硫酸钠胶束相互作用时的结构和位置。通过向溶剂中添加顺磁探针（锰离子、5-脱氧硬脂酸和12-脱氧硬脂酸）来研究穿膜肽在胶束中的定位。结果表明，该肽呈直螺旋结构，其C端深入胶束内部，N端靠近胶束表面。