通过顺磁探针研究膜结合肽和蛋白质的取向的溶液 NMR。

Institute of Chemistry/Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, A-8010 Graz, Austria.

Molecules. 2013 Jun 25;18(7):7407-35. doi: 10.3390/molecules18077407.

Many peptides and proteins are attached to or immersed in a biological membrane. In order to understand their function not only the structure but also their topology in the membrane is important. Solution NMR spectroscopy is one of the most often used approaches to determine the orientation and localization of membrane-bound peptides and proteins. Here we give an application-oriented overview on the use of paramagnetic probes for the investigation of membrane-bound peptides and proteins. The examples discussed range from the large pool of antimicrobial peptides, bacterial toxins, cell penetrating peptides to domains of larger proteins or the calcium regulating protein phospholamban. Topological information is obtained in all these examples by the use of either attached or freely mobile paramagnetic tags. For some examples information obtained from the paramagnetic probes was included in the structure determination.

许多肽和蛋白质附着或沉浸在生物膜中。为了了解它们的功能，不仅需要了解其结构，还需要了解其在膜中的拓扑结构。溶液 NMR 光谱学是确定膜结合肽和蛋白质的取向和定位的最常用方法之一。在这里，我们将提供一个面向应用的概述，介绍使用顺磁探针研究膜结合肽和蛋白质。讨论的例子包括从大量的抗菌肽、细菌毒素、细胞穿透肽到较大蛋白质的结构域或钙调节蛋白磷酸化酶。在所有这些例子中，通过使用附着或自由移动的顺磁标记物来获得拓扑信息。对于一些例子，从顺磁探针获得的信息被包含在结构确定中。