Porosk Ly, Langel Ülo
Laboratory of Drug Delivery, Institute of Technology, Faculty of Science and Technology, University of Tartu, Tartu, Estonia.
Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
Front Pharmacol. 2022 Oct 21;13:1056467. doi: 10.3389/fphar.2022.1056467. eCollection 2022.
Cell penetrating peptides (CPPs) can be broadly defined as relatively short synthetic, protein derived or chimeric peptides. Their most remarkable property is their ability to cross cell barriers and facilitate the translocation of cargo, such as drugs, nucleic acids, peptides, small molecules, dyes, and many others across the plasma membrane. Over the years there have been several approaches used, adapted, and developed for the evaluation of CPP efficacies as delivery systems, with the fluorophore attachment as the most widely used approach. It has become progressively evident, that the evaluation method, in order to lead to successful outcome, should concede with the specialties of the delivery. For characterization and assessment of CPP-cargo a combination of research tools of chemistry, physics, molecular biology, engineering, and other fields have been applied. In this review, we summarize the diverse, , and approaches used for evaluation and characterization of CPP-based cargo delivery systems.
细胞穿透肽（CPPs）可广义地定义为相对较短的合成肽、蛋白质衍生肽或嵌合肽。它们最显著的特性是能够穿越细胞屏障并促进货物（如药物、核酸、肽、小分子、染料等许多物质）跨质膜转运。多年来，已经有几种方法被用于评估CPP作为递送系统的功效，这些方法有的是直接采用，有的是经过调整和改进的，其中荧光团连接法是应用最广泛的方法。越来越明显的是，为了取得成功的结果，评估方法应符合递送的特点。为了对CPP-货物进行表征和评估，化学、物理、分子生物学、工程学和其他领域的多种研究工具都得到了应用。在这篇综述中，我们总结了用于评估和表征基于CPP的货物递送系统的各种、 和 方法。 （注：原文中“diverse, , and ”处有缺失内容）
