酵母中的磺胺类药物筛选：15种磺胺类药物在酿酒酵母中与对氨基苯甲酸竞争。
Sulfa drug screening in yeast: fifteen sulfa drugs compete with p-aminobenzoate in Saccharomyces cerevisiae.
作者信息
Castelli L A, Nguyen N P, Macreadie I G
机构信息
Biomolecular Research Institute, 343 Royal Parade, 3052, Parkville, Vic., Australia.
出版信息
FEMS Microbiol Lett. 2001 May 30;199(2):181-4. doi: 10.1111/j.1574-6968.2001.tb10671.x.
Sulfa drugs have been used as antimicrobials for decades but resistance is now a problem. For major eukaryotic pathogens, including Plasmodium and Pneumocystis, sulfa drug testing is difficult or impossible. We have shown that the eukaryote yeast can be used as a model for the study of sulfa drugs within certain parameters. Fifteen sulfa drugs inhibited yeast growth in a manner indicating competition with p-aminobenzoate (pABA). Such competition resulted from direct addition of pABA or through increased expression of the pABA synthase gene (ABZ1). The model system predicts that overexpression of the pABA synthase gene can lead to drug resistance.
磺胺类药物作为抗菌剂已使用了数十年，但耐药性现已成为一个问题。对于包括疟原虫和肺孢子菌在内的主要真核病原体，磺胺类药物检测困难或无法进行。我们已经表明，真核生物酵母可以在一定参数范围内用作研究磺胺类药物的模型。十五种磺胺类药物以表明与对氨基苯甲酸（pABA）竞争的方式抑制酵母生长。这种竞争是由直接添加pABA或通过对氨基苯甲酸合酶基因（ABZ1）表达增加引起的。该模型系统预测，对氨基苯甲酸合酶基因的过表达可导致耐药性。
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