Edman J C, Goldstein A L, Erbe J G
Department of Laboratory Medicine, University of California, San Francisco 94143-0626.
Yeast. 1993 Jun;9(6):669-75. doi: 10.1002/yea.320090613.
The Saccharomyces cerevisiae gene for para-aminobenzoate (PABA) synthase has been identified based upon its ability to confer sulfonamide resistance when present on a multicopy episomal vector. The 3840 bp DNA sequence fragment reported here contains a 2199 bp open reading frame encoding a 733 amino acid protein with similarity to the two components of PABA synthase described for prokaryotes (Escherichia coli PabA and PabB), suggesting that PABA synthase is bifunctional in yeast. The cloned sequence was confirmed to be PABA synthase by gene disruption. Chromosome gel analysis places the gene for PABA synthase on chromosome XIV.
基于酿酒酵母对氨基苯甲酸（PABA）合酶基因存在于多拷贝附加型载体上时赋予磺胺抗性的能力，该基因已被鉴定出来。本文报道的3840 bp DNA序列片段包含一个2199 bp的开放阅读框，编码一个733个氨基酸的蛋白质，该蛋白质与原核生物（大肠杆菌PabA和PabB）中描述的PABA合酶的两个组分相似，这表明PABA合酶在酵母中是双功能的。通过基因破坏证实克隆的序列是PABA合酶。染色体凝胶分析将PABA合酶基因定位在第十四条染色体上。
