Iwaki T, Kurono S, Yokose Y, Kubota K, Tamai Y, Watanabe Y
Department of Biological Resources, Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan.
Yeast. 2001 Jun;18(8):737-44. doi: 10.1002/yea.722.
The ZrGPD1 and ZrGPD2 genes encoding putative glycerol-3-phosphate dehydrogenases were isolated from the salt-tolerant yeast, Zygosaccharomyces rouxii. Both genes are homologous to GPD1 of Saccharomyces cerevisiae and are constitutively expressed in Z. rouxii cells. Putative glycerol dehydrogenase genes, ZrGCY1 and ZrGCY2, which are highly homologous to GCY1 of S. cerevisiae, were also isolated. Since the level of transcripts of ZrGCY1 and ZrGCY2 increased in Z. rouxii cells subjected to salt stress, it is suggested that the pathway of the signal transduction of salt stress controls the expression of these genes. The Accession Nos of these sequences in GenBank are as follows: ZrGPD1, AB047394; ZrGPD2, AB047395; ZrGCY1, AB047396; ZrGCY2, AB047397.
从耐盐酵母鲁氏接合酵母（Zygosaccharomyces rouxii）中分离出编码假定甘油-3-磷酸脱氢酶的ZrGPD1和ZrGPD2基因。这两个基因与酿酒酵母（Saccharomyces cerevisiae）的GPD1同源，且在鲁氏接合酵母细胞中组成型表达。还分离出了与酿酒酵母的GCY1高度同源的假定甘油脱氢酶基因ZrGCY1和ZrGCY2。由于在遭受盐胁迫的鲁氏接合酵母细胞中ZrGCY1和ZrGCY2的转录本水平增加，表明盐胁迫信号转导途径控制这些基因的表达。这些序列在GenBank中的登录号如下：ZrGPD1，AB047394；ZrGPD2，AB047395；ZrGCY1，AB047396；ZrGCY2，AB047397。
