Coetzee K, Bermes N, Krause W, Menkveld R
Department of Obstetrics and Gynaecology, Tygerberg Hospital, Tygerberg, South Africa.
Andrologia. 2001 May;33(3):159-63. doi: 10.1046/j.1439-0272.2001.00421.x.
By eliminating the human evaluation variable, it was possible to carry out an investigation into the 'true' association between normal sperm morphology outcomes assessed according to the World Health Organization guidelines and strict criteria. Two computer-assisted semen analysis systems were used, IVOS and Mika, to evaluate Diff-Quik and Papanicolaou stained slides. As expected, the mean normal sperm morphology outcomes for the World Health Organization classification evaluations were markedly higher for both the Diff-Quik (mean difference = 40.13%) and the Papanicolaou (mean difference = 32.55%) stained slides. The association between the outcomes were low for the Diff-Quik stained slides (r = 0.379) and poor for the Papanicolaou stained slides (r = 0.110). While the association achieved with the computer-assisted semen analysis systems using Diff-Quik stained slides was comparable to the association between the manual evaluations (r = 0.386), the manual evaluation of Papanicolaou stained slides produced a relatively good association (r = 0.690). Although the numbers were small, the results show the probability of poor class correlations. Approximately 40% of outcomes were incorrectly classed at a 14% (strict criteria) and 50% (World Health Organization guidelines) cut-off point for both staining methods. This study confirms the fundamental differences between the two classification systems. The results also indicate that, of the two stains used, Diff-Quik should be the preferred staining method for computer-assisted sperm morphology evaluations.
通过消除人工评估变量，得以对根据世界卫生组织指南和严格标准评估的正常精子形态结果之间的“真实”关联进行调查。使用了两种计算机辅助精液分析系统，IVOS和Mika，来评估Diff-Quik染色和巴氏染色的玻片。正如预期的那样，对于Diff-Quik染色玻片（平均差异 = 40.13%）和巴氏染色玻片（平均差异 = 32.55%），世界卫生组织分类评估的平均正常精子形态结果明显更高。Diff-Quik染色玻片的结果之间的关联较低（r = 0.379），巴氏染色玻片的关联较差（r = 0.110）。虽然使用Diff-Quik染色玻片的计算机辅助精液分析系统所实现的关联与人工评估之间的关联相当（r = 0.386），但巴氏染色玻片的人工评估产生了相对较好的关联（r = 0.690）。尽管数量较少，但结果显示了分类相关性较差的可能性。对于两种染色方法，在14%（严格标准）和50%（世界卫生组织指南）的截断点时，约40%的结果被错误分类。本研究证实了两种分类系统之间的根本差异。结果还表明，在所使用的两种染色方法中，Diff-Quik应是计算机辅助精子形态评估的首选染色方法。
