Menkveld R, Lacquet F A, Kruger T F, Lombard C J, Sanchez Sarmiento C A, de Villiers A
Department of Obstetrics and Gynaecology, Tygerberg Hospital, South Africa.
Andrologia. 1997 Jan-Feb;29(1):1-7. doi: 10.1111/j.1439-0272.1997.tb03141.x.
The purpose of this study was to investigate the effect of different staining and washing procedures on the results of human sperm morphology evaluation by manual and computerised methods. Furthermore, it was intended to find the staining and washing combination which would provide optimal readability for computer-assisted sperm morphology evaluations. In phase one, four staining methods were evaluated for smears prepared from the resulting samples following a two times washing procedure. In phase two, 20 semen samples were used to compare the Diff-Quik and Papanicolaou staining methods, following one and two washes. All manual readings, of Papanicolaou and Diff-Quik stained smears, were comparable with each other, with means between 7.3% and 7.9% normal spermatozoa. All the manual readings were also comparable to the computer readings of the Diff-Quik slides following one and two washes with means of 9.0% and 5.9%, respectively. However, due to the higher computer readings found for the Papanicolaou stained smears, with means of 13.9% and 13.5% following one and two washes, respectively, a statistically significantly difference between overall computer and manual readings was found (Wilks' Lamda, P = 0.0002). Taking all data into consideration, it could be concluded that the one wash Diff-Quik stained smears was the optimal preparation method for computerised sperm morphology evaluation, comparing favourably with manual evaluations.
本研究的目的是通过手动和计算机方法研究不同染色和洗涤程序对人类精子形态评估结果的影响。此外，旨在找到能为计算机辅助精子形态评估提供最佳可读性的染色和洗涤组合。在第一阶段，对经过两次洗涤程序后所得样本制备的涂片评估了四种染色方法。在第二阶段，使用20份精液样本比较了Diff-Quik和巴氏染色方法，分别进行一次和两次洗涤。巴氏染色和Diff-Quik染色涂片的所有手动读数彼此可比，正常精子的平均值在7.3%至7.9%之间。所有手动读数也与Diff-Quik玻片在一次和两次洗涤后的计算机读数可比，平均值分别为9.0%和5.9%。然而，由于巴氏染色涂片的计算机读数较高，一次和两次洗涤后的平均值分别为13.9%和13.5%，因此发现计算机和手动读数之间存在统计学显著差异（威尔克斯'λ，P = 0.0002）。综合所有数据，可以得出结论，一次洗涤的Diff-Quik染色涂片是计算机精子形态评估的最佳制备方法，与手动评估相比具有优势。
