Coetzee K, Kruger T F, Vandendael A, de Villiers A, Lombard C J
Department Obstetrics and Gynecology, University of Stellenbosch, Tygerberg Hospital, South Africa.
Andrologia. 1997 May-Jun;29(3):133-5. doi: 10.1111/j.1439-0272.1997.tb00306.x.
The purpose of the study was to analyse the agreement between computer analysed (Hamilton Thorne, IVOS Dimensions Version 3) normal sperm morphology and values obtained from 97 slides stained according to the Papanicolaou and Diff-Quik method. Liquefied semen samples were washed once by centrifugation and air dried smears on slides were made, which were stained according to the Papanicolaou and Diff-Quik method and analysed by computer. The paired t-test was used to assess whether any bias existed between the two methods. The limits of agreement were calculated using the Bland and Altman approach and a modification of this approach (mean-dependent limits). A significant bias of 1.6% was obtained in favour of higher normal sperm morphology percentages when using the Diff-Quik method. The standard limits of agreement were -13.4% to 16.6%, whereas the mean-dependent limits of agreement were 1.6% [5.8 + 0.6 (mean percentage normal morphology)]. Statistically, the Diff-Quik and Papanicolaou staining methods produce different normal sperm morphology profiles. These inherent differences may, therefore, require the establishment of new normal sperm morphology thresholds for male fertility, based on clinical data, when using the Diff-Quik staining method in conjunction with computerized analysis.
本研究的目的是分析计算机分析（汉密尔顿·索恩公司，IVOS Dimensions版本3）的正常精子形态与通过巴氏染色法和Diff-Quik染色法对97张涂片染色后获得的值之间的一致性。将液化精液样本离心洗涤一次，制作载玻片上的空气干燥涂片，按照巴氏染色法和Diff-Quik染色法进行染色，并通过计算机进行分析。采用配对t检验来评估两种方法之间是否存在偏差。使用布兰德-奥特曼方法及其改进方法（均值相关限）计算一致性界限。使用Diff-Quik染色法时，获得了有利于更高正常精子形态百分比的1.6%的显著偏差。一致性的标准界限为-13.4%至16.6%，而均值相关的一致性界限为1.6% [5.8 + 0.6（正常形态的平均百分比）]。从统计学角度来看，Diff-Quik染色法和巴氏染色法产生不同的正常精子形态图谱。因此，当将Diff-Quik染色法与计算机分析结合使用时，基于临床数据，这些内在差异可能需要为男性生育力建立新的正常精子形态阈值。
