Jaspars L H, Vos W, Aust G, Van Lier R A, Hamann J
Department of Pathology, Academic Hospital, Free University, Amsterdam, The Netherlands.
Tissue Antigens. 2001 Apr;57(4):325-31. doi: 10.1034/j.1399-0039.2001.057004325.x.
CD97 is a founding member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) receptors. CD97 has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to CD55. Previous studies demonstrated the expression of CD97 on activated lymphocytes, monocytes, macrophages, granulocytes, and numerous haematopoietic and nonhaematopoietic cell lines. Here, we determined the cellular distribution of human CD97 in situ by immunohistochemistry (IH) and immunofluorescence (IF). Abundant expression of CD97 was detected on all types of macrophages and dendritic cells, except for microglia. Within the lymphoid lineage, most T cells but only a few B cells express CD97. Germinal centre B cells do not express the molecule. Except for smooth muscle cells, no staining was found on other cells outside the immune system. However, analysis of a restricted set of epithelial tumors revealed CD97 expression on the malignant cells in thyroid and gastrointestinal tract cancer.
CD97是II类七跨膜（7-TM）受体的EGF-TM7家族的创始成员。CD97具有一个延伸的细胞外区域，带有几个N端表皮生长因子（EGF）样结构域，这些结构域介导与CD55的结合。先前的研究表明CD97在活化的淋巴细胞、单核细胞、巨噬细胞、粒细胞以及众多造血和非造血细胞系上表达。在此，我们通过免疫组织化学（IH）和免疫荧光（IF）确定了人CD97在体内的细胞分布。除小胶质细胞外，在所有类型的巨噬细胞和树突状细胞上均检测到CD97的大量表达。在淋巴谱系中，大多数T细胞表达CD97，但只有少数B细胞表达。生发中心B细胞不表达该分子。除平滑肌细胞外，在免疫系统以外的其他细胞上未发现染色。然而，对一组有限的上皮肿瘤的分析显示，甲状腺癌和胃肠道癌的恶性细胞上有CD97表达。
