尿嘧啶核苷和核苷酸糖基扭转角的质子磁共振研究。
A proton magnetic resonance investigation of the glycosyl torsion angle of uracil nucleosides and nucleotides.
作者信息
Schleich T, Lusebrink T R, Cross B P, Johnson N P
出版信息
Nucleic Acids Res. 1975 Apr;2(4):459-67. doi: 10.1093/nar/2.4.459.
Abstract
The use of line-shape decomposition techniques permitted the small 5-bond (5-J51') and 4-bond (4-J61') proton-proton coupling constants of a series of uracil nucleosides and nucleotides to be determined accurately. From an analysis of these coupling constants we have determined that the uracil base is in a predominantly anti conformation in aqueous solution and the mean position is not substantially altered by phosphate substitution at the 2', 3', or 5' positions, by changing the furanose stereochemistry from a ribose to a deoxyribose or an arabinose, or by an increase in temperature of 43 degree C.
摘要
线形分解技术的应用使得一系列尿嘧啶核苷和核苷酸的小的5-键（5-J51'）和4-键（4-J61'）质子-质子耦合常数得以准确测定。通过对这些耦合常数的分析，我们确定尿嘧啶碱基在水溶液中主要处于反式构象，并且在2'、3'或5'位进行磷酸取代、将呋喃糖立体化学从核糖改变为脱氧核糖或阿拉伯糖，或温度升高43℃时，平均位置基本没有改变。
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2
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3
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4
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