Effect of hypoxia, ischemia and carbon monoxide intoxication on in vivo protein synthesis in neuron and glia cell enriched fractions from rat brain.

The dynamics of changes in the in vivo amino acid incorporation into proteins of the neuron and glia cell enriched fractions of rat brain following application of a number of hypoxic conditions were investigated. The experimental models include: a) simple hypoxia, b) ischemic-hypoxic hypoxia and c) carbon monoxide intoxication. Irrespective of the experimental model used, neuronal protein synthesis was more markedly inhibited than glial, maximal inhibition being observed 2 hrs after cessation of the experimental procedure. The changes were of transient character, whereby 24 hrs after the experiment the glial synthesis appeared activated above the control value. The higher sensitivity of the neuronal protein synthesis to the applied experimental procedures produced more significant changes of the protein/total radioactivity ratio in neurons than in glia, the ratio expressing the extent of radioactive amino acid utilisation in the synthesis.