Kawamura M, Niitsu A, Nishi H, Masaki E
Department of Pharmacology (I), Jikei University School of Medicine, Tokyo, Japan.
Jpn J Pharmacol. 2001 Apr;85(4):376-81. doi: 10.1254/jjp.85.376.
We examined the effect of extracellular adenosine 5'-triphosphate (ATP) on adrenocorticotropic hormone (ACTH)- and angiotensin II-induced steroidogenesis in bovine adrenocortical fasciculata cells. The low concentration of ATP (5 microM) potentiated ACTH-induced steroidogenesis synergistically. However, the purine derivative did not affect angiotensin II-induced steroidogenesis. Although adenosine (100 microM) (a metabolite of ATP) showed a weak steroidogenic effect, it did not potentiate ACTH-induced steroidogenesis. ATP also enhanced the steroidogenesis by NaF synergistically in bovine adrenocortical cells, but did not potentiate forskolin- and dibutyryl cyclic AMP-induced steroidogenesis. The stimulating effect of ACTH on cyclic AMP production was synergistically accelerated by ATP (5 microM), which has no effect by itself on cyclic AMP formation. These results suggest that extracellular ATP affected the ACTH receptor-adenylyl cyclase coupling processes, and potentiation of steroidogenesis by ACTH ensued in bovine adrenocortical fasciculata cells.
我们研究了细胞外5'-三磷酸腺苷（ATP）对牛肾上腺皮质束状带细胞中促肾上腺皮质激素（ACTH）和血管紧张素II诱导的类固醇生成的影响。低浓度的ATP（5微摩尔）协同增强了ACTH诱导的类固醇生成。然而，这种嘌呤衍生物并不影响血管紧张素II诱导的类固醇生成。尽管腺苷（100微摩尔）（ATP的一种代谢产物）显示出较弱的类固醇生成作用，但它并没有增强ACTH诱导的类固醇生成。ATP在牛肾上腺皮质细胞中也协同增强了氟化钠诱导的类固醇生成，但没有增强福斯高林和二丁酰环磷腺苷诱导的类固醇生成。ACTH对环磷腺苷生成的刺激作用被ATP（5微摩尔）协同加速，而ATP本身对环磷腺苷的形成没有影响。这些结果表明，细胞外ATP影响了ACTH受体-腺苷酸环化酶偶联过程，并在牛肾上腺皮质束状带细胞中导致了ACTH对类固醇生成的增强作用。
