Niitsu A
Department of Internal Medicine (III), Jikei University School of Medicine, Tokyo, Japan.
Jpn J Pharmacol. 1992 Nov;60(3):269-74. doi: 10.1254/jjp.60.269.
The effect of extracellular ATP on steroidogenesis in primary cultured bovine adrenocortical fasciculata cells was investigated. I observed that in the presence of extracellular Ca2+, ATP caused a dose-dependent elevation of intracellular Ca2+ ([Ca2+]i) and induced steroidogenesis concentration- and time-dependently. However, in the absence of extracellular Ca2+, ATP had no effect on steroidogenesis. In the presence of extracellular Ca2+, calmodulin inhibitors inhibited the ATP-induced steroidogenesis, but dihydropyridine calcium channel blockers did not. Furthermore, ATP did not cause an elevation of cyclic AMP in bovine adrenocortical fasciculata cells even if extracellular Ca2+ existed. These results suggest that extracellular ATP might have an influence on bovine adrenocortical cells via the purinoceptor (P2Y) in connection with calcium mobilization, open the non-selective calcium channel and induce steroidogenesis by means of an elevation of [Ca2+]i via the calcium-calmodulin system.
研究了细胞外ATP对原代培养的牛肾上腺皮质束状带细胞类固醇生成的影响。我观察到，在细胞外Ca2+存在的情况下，ATP引起细胞内Ca2+（[Ca2+]i）呈剂量依赖性升高，并浓度和时间依赖性地诱导类固醇生成。然而，在细胞外Ca2+不存在的情况下，ATP对类固醇生成没有影响。在细胞外Ca2+存在的情况下，钙调蛋白抑制剂抑制ATP诱导的类固醇生成，但二氢吡啶类钙通道阻滞剂则没有。此外，即使细胞外Ca2+存在，ATP也不会引起牛肾上腺皮质束状带细胞中环磷酸腺苷（cAMP）升高。这些结果表明，细胞外ATP可能通过嘌呤受体（P2Y）与钙动员相关，打开非选择性钙通道，并通过钙-钙调蛋白系统升高[Ca2+]i来影响牛肾上腺皮质细胞，进而诱导类固醇生成。
