Kawamura M, Matsui T, Niitsu A, Kondo T, Ohno Y, Nakamichi N
Department of Pharmacology I, Jikei University School of Medicine, Tokyo, Japan.
Jpn J Pharmacol. 1991 Aug;56(4):543-5. doi: 10.1254/jjp.56.543.
Effects of ATP and other purine derivatives on steroidogenesis in primary cultured bovine adrenocortical fasciculata cells were examined. At concentrations higher than 1 microM, ATP showed a potent stimulative effect on the cortisol production of the cells. The potency order of the steroidogenic effect of the tested purine derivatives was ATP greater than ADP much greater than adenosine much greater than AMP. alpha,beta-Methylene ATP had no stimulative effect on the steroidogenesis at concentrations as high as 1 mM. Theophylline did not antagonize the steroidogenic effect of ATP. These results suggest that bovine adrenocortical fasciculata cells possess the P2y purinoceptors that are linked to steroidogenesis.
研究了ATP及其他嘌呤衍生物对原代培养的牛肾上腺皮质束状带细胞类固醇生成的影响。当浓度高于1微摩尔时，ATP对细胞皮质醇生成表现出强烈的刺激作用。所测试的嘌呤衍生物的类固醇生成效应的效力顺序为：ATP＞ADP＞＞腺苷＞＞AMP。α,β-亚甲基ATP在高达1毫摩尔的浓度下对类固醇生成没有刺激作用。茶碱不拮抗ATP的类固醇生成效应。这些结果表明，牛肾上腺皮质束状带细胞具有与类固醇生成相关的P2y嘌呤受体。
