Kirsch C, Logemann E, Lippok B, Schmelzer E, Hahlbrock K
Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Carl-von-Linne-Weg 10, D-50829 Köln, Germany.
Plant J. 2001 Apr;26(2):217-27. doi: 10.1046/j.1365-313x.2001.01015.x.
Within the complex signalling network from pathogen-derived elicitor perception to defense-related gene activation, some immediate-early responding genes may have pivotal roles in downstream transcriptional regulation. We have identified the parsley (Petroselinum crispum) ELI17 gene as a particularly fast-responding gene possessing a new type of W box-containing, elicitor-responsive promoter element, E17. Highly selective E17-mediated reporter gene expression at pathogen infection sites in transgenic Arabidopsis thaliana plants demonstrated the potential of this promoter element for designing new strategies in resistance breeding as well as for further analysis of the early components of defense-related gene activation mechanisms. The protein encoded by the ELI17 gene exhibits various structural characteristics of established transcription factors and is designated as a CMPG protein according to the first four strictly conserved amino acids defining a newly emerging class of plant-specific proteins.
在从病原体衍生激发子感知到防御相关基因激活的复杂信号网络中，一些早期快速响应基因可能在下游转录调控中起关键作用。我们已将欧芹（Petroselinum crispum）的ELI17基因鉴定为一个响应特别迅速的基因，其具有一种新型的含W盒的激发子响应启动子元件E17。在转基因拟南芥植物的病原体感染位点，高度选择性的E17介导的报告基因表达证明了该启动子元件在抗性育种新策略设计以及防御相关基因激活机制早期成分进一步分析方面的潜力。ELI17基因编码的蛋白质展现出已确定的转录因子的各种结构特征，并根据定义一类新出现的植物特异性蛋白质的前四个严格保守氨基酸被指定为CMPG蛋白。
