van de Löcht U, Meier I, Hahlbrock K, Somssich I E
Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG.
EMBO J. 1990 Sep;9(9):2945-50. doi: 10.1002/j.1460-2075.1990.tb07486.x.
We describe the nucleotide sequence and some structural characteristics of a single copy gene encoding pathogenesis-related protein 2 (PR2) in parsley (Petroselinum crispum). Transcriptional activation of this gene in cultured parsley cells treated with fungal elicitor leads to a rapid, large and transient accumulation of PR2 mRNA. The deduced PR2 protein belongs to a novel class of evolutionarily conserved polypeptides which are closely related to disease resistance in plants. Functional analysis of a series of truncated PR2 promoter fusions with the beta-glucuronidase reporter gene, using parsley protoplasts for transient expression studies, identified a 5' upstream element between positions -168 and -52 necessary for strong elicitor responsiveness. This small promoter fragment is active in conjunction with its own TATA box region as well as with the corresponding region from a heterologous promoter. The PR2 regulatory region exhibits no sequence similarity to any other elicitor-responsive promoter known to date.
我们描述了在欧芹（Petroselinum crispum）中编码病程相关蛋白2（PR2）的单拷贝基因的核苷酸序列和一些结构特征。在用真菌激发子处理的培养欧芹细胞中，该基因的转录激活导致PR2 mRNA迅速、大量且短暂地积累。推导的PR2蛋白属于一类新的进化保守多肽，与植物抗病性密切相关。使用欧芹原生质体进行瞬时表达研究，对一系列与β-葡萄糖醛酸酶报告基因融合的截短PR2启动子进行功能分析，确定了-168至-52位之间的一个5'上游元件，它是强激发子反应性所必需的。这个小的启动子片段与其自身的TATA盒区域以及来自异源启动子的相应区域一起具有活性。PR2调控区域与迄今已知的任何其他激发子反应性启动子均无序列相似性。
