Alvarez Angela M, Neubeck Stefan, Parra Sergio, Markert Udo R, Cadavid Angela P
Reproduction Group, Department of Microbiology and Parasitology, School of Medicine, Universidad de Antioquia, Medellín, Colombia.
Department of Obstetrics, University Hospital Jena, Jena, Germany.
J Hum Reprod Sci. 2017 Jan-Mar;10(1):10-17. doi: 10.4103/0974-1208.204018.
Antiphospholipid antibodies (aPL) are related with a high risk of pregnancy morbidity (PM) and also of vascular thrombosis. On the basis of recent studies, we expect that in women with PM associated with antiphospholipid syndrome (APS), further factors may be deregulated and involved in pathophysiology of the disease. Such factors may have the potential to become novel biomarkers for APS and its stages.
Descriptive study from a recurrent pregnancy loss program.
To study the protein expression in sera from women with PM with or without aPL.
Protein profiles were determined by surface enhanced laser desorption and ionization - time of flight mass spectrometry (SELDI-TOF MS) in the serum samples from women with PM, 10 of them with aPL and 12 without aPL. On the basis of the mass-to-charge ratio () of the protein, signals differentially expressed between the two groups were compared with data banks to approach candidate proteins.
To determine the differential expression of each protein, a no paired -test was performed using Ciphergen Express Client 3.1 software.
SELDI-TOF analysis makes it possible to discriminate between several proteins in women with PM with and without aPL, although it does not allow protein identification. Nine proteins were found in significantly higher levels in aPL-positive women.
The results underline that further factors beyond autoantibodies are involved in PM associated with APS and might lead to the development of new biomarkers.
抗磷脂抗体（aPL）与妊娠并发症（PM）及血管血栓形成的高风险相关。基于最近的研究，我们预期在患有与抗磷脂综合征（APS）相关的PM的女性中，可能存在其他失调的因素参与该疾病的病理生理过程。这些因素有可能成为APS及其分期的新型生物标志物。
一项来自复发性流产项目的描述性研究。
研究患有或不患有aPL的PM女性血清中的蛋白质表达情况。
采用表面增强激光解吸电离飞行时间质谱（SELDI-TOF MS）测定PM女性血清样本中的蛋白质谱，其中10名患有aPL，12名不患有aPL。根据蛋白质的质荷比（），将两组间差异表达的信号与数据库进行比对以寻找候选蛋白质。
使用Ciphergen Express Client 3.1软件进行非配对检验以确定每种蛋白质的差异表达。
SELDI-TOF分析能够区分患有和不患有aPL的PM女性中的几种蛋白质，尽管无法鉴定蛋白质。在aPL阳性女性中发现9种蛋白质水平显著更高。
结果强调，除自身抗体外，还有其他因素参与了与APS相关的PM，可能会促成新生物标志物的开发。
