Aich S, Manna C K
Department of Zoology, University of Kalyani, West Bengal, India.
Acta Biol Hung. 2001;52(1):105-16. doi: 10.1556/ABiol.52.2001.1.10.
The results of the present study indicate the antispermatogenic activity of Busulphan or Myleran (1,4-dimethane-sulphonoxy butane) on the testicular tissue of adult male Indian house rat, Rattus rattus. Single oral dose of Busulphan (10 mg/Kg body weight) was administered and its activity was noticed at 10, 40, 70 and 100 days of posttreated animals. Histological observation and quantitative histological study indicates no major alteration in the relative percentages of primary spermatocytes, spermatid and Sertoli cells at 10 days of posttreatment. But there was a gradual decrease in the seminiferous tubular diameter at 40 and 70 days of post treated groups. However, the Leydig and Sertoli cells morphology and number remained normal in all the treatment groups. At 40 days, the normal cellular associations in all the tubules were disrupted. The tubules constituted only spermatogonia, Sertoli cells and some zygotene spermatocytes. At 70 days, repopulation of Type A, Type B spermatogonia, resting and zygotene spermatocytes occurred at this stage. The tubules were still devoid of pachytene spermatocytes, spermatid and spermatozoa. At 100 days, active spermatogenesis was observed in majority of the tubules. The various types of germ cell population were regaining towards normalcy. Histochemical studies clearly revealed that due to busulphan administration there was no major alteration in the intensities of some key enzymes (i.e. delta5 3beta-HSDH and 17beta-HSDH) involved in the biosynthesis of steroid hormones. Only the acid phosphatase activity was slightly depressed within the 40th and 70th days of posttreatment. Sudanophilic lipid materials increased in the interstitium of all the busulphan post treated groups. The changes which were noticed due to busulphan treatment regained normalcy at 100 days of post treated animals. The mode of action of Busulphan on the testicular tissue of adult Indian house rat (Rattus rattus) has been pointed out and discussed.
本研究结果表明，白消安（或马利兰，即1,4 - 二甲烷磺酰氧基丁烷）对成年雄性印度家鼠（褐家鼠）的睾丸组织具有抗生精活性。给予单次口服剂量的白消安（10毫克/千克体重），并在处理后的动物第10、40、70和100天观察其活性。组织学观察和定量组织学研究表明，处理后第10天，初级精母细胞、精子细胞和支持细胞的相对百分比无重大变化。但处理后第40天和70天，生精小管直径逐渐减小。然而，所有处理组中的睾丸间质细胞和支持细胞的形态和数量保持正常。在第40天，所有小管中的正常细胞关联被破坏。小管中仅含有精原细胞、支持细胞和一些偶线期精母细胞。在第70天，此时A型、B型精原细胞、静止期和偶线期精母细胞重新出现。小管中仍然没有粗线期精母细胞、精子细胞和精子。在第100天，大多数小管中观察到活跃的精子发生。各种类型的生殖细胞群体正恢复正常。组织化学研究清楚地表明，由于给予白消安，参与类固醇激素生物合成的一些关键酶（即δ5 3β - 羟类固醇脱氢酶和17β - 羟类固醇脱氢酶）的强度没有重大变化。仅在处理后第40天和70天内酸性磷酸酶活性略有降低。在所有白消安处理组的间质中嗜苏丹脂质物质增加。在处理后第100天，由于白消安处理而观察到的变化恢复正常。已指出并讨论了白消安对成年印度家鼠（褐家鼠）睾丸组织的作用方式。
