正常、早期和晚期骨关节炎绵羊颞下颌关节中硫酸软骨素、二糖和透明质酸的定量分析
Quantitation of chondroitin-sulfates, disaccharides and hyaluronan in normal, early and advanced osteoarthritic sheep temporomandibular joints.
作者信息
Ishimaru J I, Ogi N, Mizuno S, Goss A N
机构信息
Department of Oral and Maxillofacial Surgery, Gifu Prefectural Gifu Hospital, Japan.
出版信息
Osteoarthritis Cartilage. 2001 May;9(4):365-70. doi: 10.1053/joca.2000.0397.
OBJECTIVE
To determine the relationship between synovial fluid, chondroitin sulfate disaccharide and hyaluronic acid to differing degrees of experimental temporomandibular joint (TMJ) osteoarthritis (OA).
DESIGN
Twenty-four merino sheep were divided into three groups and had different TMJ surgical procedures to produce OA. Group I; control (six sheep), Group II; disc perforation (nine sheep) and Group III; disc perforation and articular damage (nine sheep). Synovial fluid was collected initially and at sacrifice at 3 months. Chondroitin 4-sulfate, chondroitin 6-sulfate and hyaluronic acid were measured and correlated to the OA histologic score.
RESULTS
The chondroitin-sulfate levels were significantly increased (Group I to Group II P< 0.001; Group I to Group III P< 0.001), the hyaluronic acid levels decreased (Group I to Group II P< 0.01; Group I to Group III P< 0.01) with the increasing OA score.
CONCLUSION
Chondroitin-sulfate and hyaluronic acid show a correlation with surgically created TMJ osteoarthritis in sheep model.
目的
确定滑液、硫酸软骨素二糖和透明质酸与不同程度的实验性颞下颌关节（TMJ）骨关节炎（OA）之间的关系。
设计
将24只美利奴绵羊分为三组，进行不同的颞下颌关节手术以诱发骨关节炎。第一组为对照组（6只绵羊），第二组为盘穿孔组（9只绵羊），第三组为盘穿孔和关节损伤组（9只绵羊）。在实验开始时以及3个月处死时采集滑液。测量硫酸软骨素4、硫酸软骨素6和透明质酸的含量，并将其与骨关节炎组织学评分进行关联分析。
结果
随着骨关节炎评分的增加，硫酸软骨素水平显著升高（第一组与第二组比较，P<0.001；第一组与第三组比较，P<0.001），透明质酸水平降低（第一组与第二组比较，P<0.01；第一组与第三组比较，P<0.01）。
结论
在绵羊模型中，硫酸软骨素和透明质酸与手术诱发的颞下颌关节骨关节炎存在相关性。
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