[胶质细胞成熟因子研究综述及人和大鼠胶质细胞成熟因子-γ（GMFG）cDNA的克隆]

[Review of the research of glia maturation factor and cloning of human and rat glia maturation factor-gamma (GMFG) cDNA].

作者信息

Asai K

机构信息

Department of Bioregulation Research, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.

出版信息

Nihon Shinkei Seishin Yakurigaku Zasshi. 2001 Feb;21(1):15-20.

PMID:11400321

Abstract

Glia maturation factor-beta (GMFB) is a 17-kDa protein that was initially identified as a growth and differentiation factor acting on neurons as well as glia in the vertebrate brain. We isolated human and rat glia maturation factor-gamma (GMFG) cDNA and examined the tissue distribution of GMFG in human and rat by Northern blots and Western blots. Sequence analysis of the entire cDNA revealed an open reading frame of 426 nucleotides with a deduced protein of 142 amino acid residues. The deduced amino acid sequence of its putative product is highly homologous to GMFB. Northern blot analysis indicated that a 0.9 kb mRNA is predominantly expressed in rat thymus, testis, and spleen. In comparison with GMFB, the current study demonstrated that the tissue distribution of GMFG is not the same as that of GMFB, and GMFG is predominantly in proliferative and differentiative organs.

摘要

胶质细胞成熟因子-β（GMFB）是一种17 kDa的蛋白质，最初被鉴定为一种生长和分化因子，作用于脊椎动物大脑中的神经元和胶质细胞。我们分离了人和大鼠的胶质细胞成熟因子-γ（GMFG）cDNA，并通过Northern印迹和Western印迹检测了GMFG在人和大鼠中的组织分布。对整个cDNA的序列分析揭示了一个426个核苷酸的开放阅读框，推导的蛋白质有142个氨基酸残基。其推定产物的推导氨基酸序列与GMFB高度同源。Northern印迹分析表明，一个0.9 kb的mRNA主要在大鼠胸腺、睾丸和脾脏中表达。与GMFB相比，当前研究表明GMFG的组织分布与GMFB不同，且GMFG主要存在于增殖和分化器官中。