Inagaki Masaaki, Aoyama Mineyoshi, Sobue Kazuya, Yamamoto Naoki, Morishima Tetsuro, Moriyama Akihiko, Katsuya Hirotada, Asai Kiyofumi
Department of Molecular Neurobiology, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho, Nagoya 467-8601, Japan.
Biochim Biophys Acta. 2004 Feb 24;1670(3):208-16. doi: 10.1016/j.bbagen.2003.12.006.
We developed sensitive and specific two-site enzyme immunoassays (EIA) for glia maturation factor beta (GMFB) and gamma (GMFG) using specific antibodies raised in rabbits. These assay systems enabled us to identify GMFB and GMFG (GMFs) in both human and rat samples and they were used to investigate the tissue distribution and serum concentrations of human and rat GMFs. In the case of rat, relatively high levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon. Higher levels of GMFG were found in the thymus, spleen and colon. The distribution of GMFs in human was similar to that in rat. In the rat, the maximum serum concentration of GMFG was at 4 weeks of age. The decrease in its level was rapid for the first 30 days of life in both sexes. On the other hand, the concentration of GMFB in serum did not change significantly with age. Similarly, in human, the concentration of GMFG in serum was highest in the 21-30-year-old group and began to decrease rapidly in the 30-year-old group. In contrast, the concentration of GMFB did not change significantly during this period. No significant sex differences in the serum levels of GMFs were observed in human and rat. The present EIA systems are sufficiently sensitive for studying GMFs in human and rat organs.
我们使用兔源特异性抗体开发了用于检测胶质细胞成熟因子β（GMFB）和γ（GMFG）的灵敏且特异的双位点酶免疫测定法（EIA）。这些测定系统使我们能够在人和大鼠样本中鉴定出GMFB和GMFG（GMFs），并用于研究人和大鼠GMFs的组织分布及血清浓度。就大鼠而言，在中枢神经系统（脊髓除外）、胸腺和结肠中发现GMFB水平相对较高。在胸腺、脾脏和结肠中发现GMFG水平较高。GMFs在人体内的分布与在大鼠体内相似。在大鼠中，GMFG的最高血清浓度出现在4周龄时。在出生后的前30天，两性的GMFG水平下降迅速。另一方面，血清中GMFB的浓度并未随年龄显著变化。同样，在人类中，血清中GMFG的浓度在21 - 30岁组最高，并在30岁组开始迅速下降。相比之下，在此期间GMFB的浓度没有显著变化。在人和大鼠中均未观察到GMFs血清水平存在显著的性别差异。目前的EIA系统对于研究人和大鼠器官中的GMFs具有足够的灵敏度。
