Kapley A, Purohit H J
Environ Sci Pollut Res Int. 2001;8(2):89-90. doi: 10.1007/BF02987299.
A simple and rapid protocol has been developed for monitoring of the phenol degrading population in the environmental samples. PCR protocol uses the total DNA prepared from the samples as a template in the amplification reaction. Primers have been designed from the sequence data for Dmp and Phe operon encoding multi-component and single component phenol oxidizing system, respectively. The phenol degrading genotype in various samples have been demonstrated using the developed PCR protocol.
已开发出一种简单快速的方案，用于监测环境样品中降解苯酚的菌群。聚合酶链反应（PCR）方案在扩增反应中使用从样品中制备的总DNA作为模板。分别根据编码多组分和单组分苯酚氧化系统的Dmp和Phe操纵子的序列数据设计了引物。已使用所开发的PCR方案证实了各种样品中降解苯酚的基因型。
