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生物修复中的基因组工具。

Genomic tools in bioremediation.

机构信息

Environmental Genomics Division, National Environmental Engineering Research Institute, CSIR, Nehru Marg, Nagpur 440 020 India.

出版信息

Indian J Microbiol. 2009 Jun;49(2):108-13. doi: 10.1007/s12088-009-0012-2. Epub 2009 Mar 14.

Abstract

Bioremediation is a process that uses microorganisms or their enzymes to remove pollutants from the environment. Generally, bioremediation technologies can be classified as in situ or ex situ. In situ bioremediation involves treating the contaminated material at the site while ex situ involves the removal of the contaminated material to be treated elsewhere. Like so much else in biology, the ease and availability of genomic data has created a new level of understanding this system. Bioremediation capabilities of the microbial population can be analyzed; not only by physiological parameters, but also by the use of genomic tools, and efficient remediation strategies can be planned. PCR and DNA- or oligonucleotide-based microarray technology is a powerful functional genomics tool that allows researchers to view the physiology of a living cell from a comprehensive and dynamic molecular perspective. This paper explores the use of such tools in bioremediation process.

摘要

生物修复是一种利用微生物或其酶来去除环境污染物的过程。一般来说,生物修复技术可以分为原位和异位。原位生物修复涉及在污染地点处理受污染的材料,而异位则涉及将受污染的材料移走,在其他地方进行处理。就像生物学中的许多其他方面一样,基因组数据的易得性和可用性为我们理解这一系统创造了一个新的水平。可以通过生理参数,也可以通过基因组工具来分析微生物种群的生物修复能力,从而可以规划有效的修复策略。聚合酶链式反应(PCR)和基于 DNA 或寡核苷酸的微阵列技术是一种强大的功能基因组学工具,它使研究人员能够从全面和动态的分子角度观察活细胞的生理学。本文探讨了这些工具在生物修复过程中的应用。

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