Lutter E, Lewenza S, Dennis J J, Visser M B, Sokol P A
Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta, Canada T2N 4N1.
Infect Immun. 2001 Jul;69(7):4661-6. doi: 10.1128/IAI.69.7.4661-4666.2001.
The distribution of quorum-sensing genes among strains from seven genomovars of the Burkholderia cepacia complex was examined by PCR. cepR and cepI were amplified from B. cepacia genomovars I and III, B. stabilis, and B. vietnamiensis. cepR was also amplified from B. multivorans and B. cepacia genomovar VI. bviIR were amplified from B. vietnamiensis. All genomovars produced N-octanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone. B. vietnamiensis and B. cepacia genomovar VII produced additional N-acyl-L-homoserine lactones.
通过聚合酶链反应（PCR）检测了洋葱伯克霍尔德菌复合体七个基因组变种菌株中群体感应基因的分布情况。cepR和cepI基因在洋葱伯克霍尔德菌基因组变种I和III、稳定伯克霍尔德菌及越南伯克霍尔德菌中得到扩增。cepR基因也在多食伯克霍尔德菌和洋葱伯克霍尔德菌基因组变种VI中得到扩增。bviIR基因在越南伯克霍尔德菌中得到扩增。所有基因组变种均产生N-辛酰基-L-高丝氨酸内酯和N-己酰基-L-高丝氨酸内酯。越南伯克霍尔德菌和洋葱伯克霍尔德菌基因组变种VII还产生了其他N-酰基-L-高丝氨酸内酯。
