霍乱弧菌tolC是胆汁抗性和定殖所必需的。
Vibrio cholerae tolC is required for bile resistance and colonization.
作者信息
Bina J E, Mekalanos J J
机构信息
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
出版信息
Infect Immun. 2001 Jul;69(7):4681-5. doi: 10.1128/IAI.69.7.4681-4685.2001.
Abstract
TolC and its homologues are outer membrane proteins that are essential for the transport of many molecules across the cell envelope. In this study we characterized the gene encoding Vibrio cholerae TolC. V. cholerae tolC mutants failed to secrete the RTX cytotoxin, were hypersensitive to antimicrobial agents, and were deficient in intestinal colonization.
摘要
TolC及其同源物是外膜蛋白，对于许多分子跨细胞膜转运至关重要。在本研究中，我们对编码霍乱弧菌TolC的基因进行了表征。霍乱弧菌tolC突变体无法分泌RTX细胞毒素，对抗菌剂高度敏感，并且在肠道定殖方面存在缺陷。
相似文献
1
Vibrio cholerae tolC is required for bile resistance and colonization.霍乱弧菌tolC是胆汁抗性和定殖所必需的。
Infect Immun. 2001 Jul;69(7):4681-5. doi: 10.1128/IAI.69.7.4681-4685.2001.
2
TolC but not AcrB is essential for multidrug-resistant Salmonella enterica serotype Typhimurium colonization of chicks.对于多重耐药的肠炎沙门氏菌鼠伤寒血清型在雏鸡体内的定殖，TolC而非AcrB是必需的。
J Antimicrob Chemother. 2005 May;55(5):707-12. doi: 10.1093/jac/dki091. Epub 2005 Apr 6.
3
Effect of bile on the cell surface permeability barrier and efflux system of Vibrio cholerae.胆汁对霍乱弧菌细胞表面渗透屏障和外排系统的影响。
J Bacteriol. 2004 Oct;186(20):6809-14. doi: 10.1128/JB.186.20.6809-6814.2004.
4
Characterization of the Vibrio cholerae vexAB and vexCD efflux systems.霍乱弧菌vexAB和vexCD外排系统的特性分析。
Arch Microbiol. 2006 Sep;186(3):171-81. doi: 10.1007/s00203-006-0133-5. Epub 2006 Jun 28.
5
The Vibrio cholerae ToxR-regulated porin OmpU confers resistance to antimicrobial peptides.霍乱弧菌ToxR调控的孔蛋白OmpU赋予对抗菌肽的抗性。
Infect Immun. 2004 Jun;72(6):3577-83. doi: 10.1128/IAI.72.6.3577-3583.2004.
6
Vibrio cholerae strains with mutations in an atypical type I secretion system accumulate RTX toxin intracellularly.在非典型I型分泌系统中发生突变的霍乱弧菌菌株会在细胞内积累RTX毒素。
J Bacteriol. 2004 Dec;186(23):8137-43. doi: 10.1128/JB.186.23.8137-8143.2004.
7
Altered expression of the ToxR-regulated porins OmpU and OmpT diminishes Vibrio cholerae bile resistance, virulence factor expression, and intestinal colonization.由ToxR调控的孔蛋白OmpU和OmpT的表达改变会降低霍乱弧菌的胆汁抗性、毒力因子表达及肠道定殖能力。
Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):10220-4. doi: 10.1073/pnas.170219997.
8
The virulence regulatory protein ToxR mediates enhanced bile resistance in Vibrio cholerae and other pathogenic Vibrio species.毒力调节蛋白ToxR介导霍乱弧菌及其他致病性弧菌对胆汁抵抗力的增强。
Infect Immun. 2000 Mar;68(3):1491-7. doi: 10.1128/IAI.68.3.1491-1497.2000.
9
The α-barrel tip region of Escherichia coli TolC homologs of Vibrio vulnificus interacts with the MacA protein to form the functional macrolide-specific efflux pump MacAB-TolC.α-桶尖端区域的弗氏弧菌大肠杆菌 TolC 同源物与 MacA 蛋白相互作用，形成功能性大环内酯类特异性外排泵 MacAB-TolC。
J Microbiol. 2013 Apr;51(2):154-9. doi: 10.1007/s12275-013-2699-3. Epub 2013 Apr 27.
10
Need for TolC, an Escherichia coli outer membrane protein, in the secretion of heat-stable enterotoxin I across the outer membrane.大肠杆菌外膜蛋白TolC在热稳定肠毒素I跨外膜分泌中的作用。
Microb Pathog. 1998 Sep;25(3):111-20. doi: 10.1006/mpat.1998.0211.
引用本文的文献
1
The carRS-ompV-virK operon of Vibrio cholerae senses antimicrobial peptides and activates the expression of multiple resistance systems.霍乱弧菌的carRS-ompV-virK操纵子可感知抗菌肽并激活多种抗性系统的表达。
Sci Rep. 2025 Apr 21;15(1):13686. doi: 10.1038/s41598-025-98217-3.
2
DNA repair is essential for growth on thiosulfate-citrate-bile salts-sucrose (TCBS) medium.DNA修复对于在硫代硫酸盐-柠檬酸盐-胆盐-蔗糖（TCBS）培养基上生长至关重要。
J Bacteriol. 2025 Apr 17;207(4):e0000425. doi: 10.1128/jb.00004-25. Epub 2025 Mar 26.
3
DNA repair is essential for growth on Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Medium.DNA修复对于在硫代硫酸盐-柠檬酸盐-胆盐-蔗糖（TCBS）培养基上生长至关重要。
bioRxiv. 2025 Jan 11:2025.01.10.632459. doi: 10.1101/2025.01.10.632459.
4
Unveiling the impact of TolC efflux protein on host tissue adherence, complement evasion, and diagnostic potential.揭示 TolC 外排蛋白对宿主组织黏附、补体逃避和诊断潜力的影响。
Infect Immun. 2024 Nov 12;92(11):e0041924. doi: 10.1128/iai.00419-24. Epub 2024 Oct 11.
5
Vibrio cholerae RND efflux systems: mediators of stress responses, colonization and pathogenesis.霍乱弧菌 RND 外排系统：应激反应、定植和发病机制的中介。
Front Cell Infect Microbiol. 2023 May 15;13:1203487. doi: 10.3389/fcimb.2023.1203487. eCollection 2023.
6
Vibrio cholerae Alkalizes Its Environment via Citrate Metabolism to Inhibit Enteric Growth .霍乱弧菌通过柠檬酸盐代谢碱化其环境以抑制肠道生长。
Microbiol Spectr. 2023 Mar 14;11(2):e0491722. doi: 10.1128/spectrum.04917-22.
7
Stress Responses in Pathogenic Vibrios and Their Role in Host and Environmental Survival.病原性弧菌的应激反应及其在宿主和环境生存中的作用。
Adv Exp Med Biol. 2023;1404:213-232. doi: 10.1007/978-3-031-22997-8_11.
8
A systematic review on omics data (metagenomics, metatranscriptomics, and metabolomics) in the role of microbiome in gallbladder disease.关于组学数据（宏基因组学、宏转录组学和代谢组学）在微生物群在胆囊疾病中的作用的系统综述。
Front Physiol. 2022 Aug 30;13:888233. doi: 10.3389/fphys.2022.888233. eCollection 2022.
9
Haloalkaline Lipase from Bacillus flexus PU2 Efficiently Inhibits Biofilm Formation of Aquatic Pathogen Vibrio parahaemolyticus.粘弹性芽孢杆菌来源的 haloalkaline 脂肪酶高效抑制水产病原菌副溶血弧菌生物膜形成。
Probiotics Antimicrob Proteins. 2022 Aug;14(4):664-674. doi: 10.1007/s12602-022-09908-6. Epub 2022 Mar 14.
10
Vibrio cholerae TolC Is Required for Expression of the ToxR Regulon.霍乱弧菌 TolC 是毒力调节子表达所必需的。
Infect Immun. 2021 Sep 16;89(10):e0024221. doi: 10.1128/IAI.00242-21. Epub 2021 Jul 26.
本文引用的文献
1
In vivo covalent cross-linking of cellular actin by the Vibrio cholerae RTX toxin.霍乱弧菌RTX毒素对细胞肌动蛋白的体内共价交联作用。
EMBO J. 2000 Oct 16;19(20):5315-23. doi: 10.1093/emboj/19.20.5315.
2
DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae.霍乱病原体霍乱弧菌两条染色体的DNA序列。
Nature. 2000 Aug 3;406(6795):477-83. doi: 10.1038/35020000.
3
Altered expression of the ToxR-regulated porins OmpU and OmpT diminishes Vibrio cholerae bile resistance, virulence factor expression, and intestinal colonization.由ToxR调控的孔蛋白OmpU和OmpT的表达改变会降低霍乱弧菌的胆汁抗性、毒力因子表达及肠道定殖能力。
Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):10220-4. doi: 10.1073/pnas.170219997.
4
Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export.细菌膜蛋白TolC的晶体结构对多药外排和蛋白质输出至关重要。
Nature. 2000 Jun 22;405(6789):914-9. doi: 10.1038/35016007.
5
The virulence regulatory protein ToxR mediates enhanced bile resistance in Vibrio cholerae and other pathogenic Vibrio species.毒力调节蛋白ToxR介导霍乱弧菌及其他致病性弧菌对胆汁抵抗力的增强。
Infect Immun. 2000 Mar;68(3):1491-7. doi: 10.1128/IAI.68.3.1491-1497.2000.
6
Helicobacter pylori uptake and efflux: basis for intrinsic susceptibility to antibiotics in vitro.幽门螺杆菌的摄取与外排：体外对抗生素内在易感性的基础
Antimicrob Agents Chemother. 2000 Feb;44(2):248-54. doi: 10.1128/AAC.44.2.248-254.2000.
7
Bile acid concentrations, cytotoxicity, and pH of fecal water from patients with colorectal adenomas.大肠腺瘤患者粪便水的胆汁酸浓度、细胞毒性及pH值
Dig Dis Sci. 1999 Nov;44(11):2218-25. doi: 10.1023/a:1026644418142.
8
Cholera, 1998.霍乱，1998年。
Wkly Epidemiol Rec. 1999 Aug 6;74(31):257-9.
9
Environmental signals modulate ToxT-dependent virulence factor expression in Vibrio cholerae.环境信号调节霍乱弧菌中ToxT依赖性毒力因子的表达。
J Bacteriol. 1999 Mar;181(5):1508-14. doi: 10.1128/JB.181.5.1508-1514.1999.
10
Identification of a vibrio cholerae RTX toxin gene cluster that is tightly linked to the cholera toxin prophage.鉴定与霍乱毒素原噬菌体紧密相连的霍乱弧菌RTX毒素基因簇。
Proc Natl Acad Sci U S A. 1999 Feb 2;96(3):1071-6. doi: 10.1073/pnas.96.3.1071.
Zotero 插件