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使用废弃灌溉水进行苜蓿芽的微生物分析。

Use of spent irrigation water for microbiological analysis of alfalfa sprouts.

作者信息

Fu T, Stewart D, Reineke K, Ulaszek J, Schlesser J, Tortorello M

机构信息

US Food and Drug Administration, National Center for Food Safety and Technology, Summit-Argo, Illinois 60501, USA.

出版信息

J Food Prot. 2001 Jun;64(6):802-6. doi: 10.4315/0362-028x-64.6.802.

DOI:10.4315/0362-028x-64.6.802
PMID:11403129
Abstract

Numerous outbreaks of foodborne illness have been linked to the consumption of raw sprouts. Sprout producers have been advised by the Food and Drug Administration to include microbiological testing of spent irrigation water during production as part of an overall strategy to enhance the safety of sprouts. Alfalfa sprouts and irrigation water were analyzed to show the feasibility of using irrigation water for monitoring the microbiological safety of sprouts. Sprouts and water were produced and harvested from both commercial-scale (rotary drum) and consumer-scale (glass jars) equipment. Rapid increases of aerobic mesophiles occurred during the first 24 h of sprouting, with maximum levels achieved after 48 to 72 h. The counts in irrigation water were on average within approximately 1 log of their respective counts in the sprouts. Similar results were obtained for analysis of Escherichia coli O157:H7 in irrigation water and sprouts grown from artificially inoculated seeds. Testing of spent irrigation water indicated the contamination status of alfalfa sprouts grown from seeds associated with outbreaks of Salmonella infection.

摘要

许多食源性疾病的爆发都与食用生豆芽有关。美国食品药品监督管理局已建议豆芽生产商将生产过程中对用过的灌溉水进行微生物检测作为提高豆芽安全性的整体策略的一部分。对苜蓿芽和灌溉水进行了分析,以证明利用灌溉水监测豆芽微生物安全性的可行性。豆芽和水分别从商业规模(转鼓式)和消费者规模(玻璃罐)设备中生产和收获。在发芽的最初24小时内,需氧嗜温菌迅速增加,在48至72小时后达到最高水平。灌溉水中的菌数平均比豆芽中各自的菌数低约1个对数级。对于灌溉水和从人工接种种子长出的豆芽中的大肠杆菌O157:H7分析,也得到了类似结果。对用过的灌溉水进行检测表明了与沙门氏菌感染爆发相关的种子所生长的苜蓿芽的污染状况。

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引用本文的文献

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Taxonomic and Functional Shifts in the Sprout Spent Irrigation Water Microbiome in Response to Contamination of Alfalfa Seeds.发芽废灌溉水微生物组在受到苜蓿种子污染时的分类和功能变化。
Appl Environ Microbiol. 2021 Jan 15;87(3). doi: 10.1128/AEM.01811-20.
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Risk Assessment of Salmonellosis from Consumption of Alfalfa Sprouts and Evaluation of the Public Health Impact of Sprout Seed Treatment and Spent Irrigation Water Testing.
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Risk Anal. 2018 Aug;38(8):1738-1757. doi: 10.1111/risa.12964. Epub 2018 Jan 16.
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Survival of murine norovirus, Tulane virus, and hepatitis A virus on alfalfa seeds and sprouts during storage and germination.苜蓿种子和芽菜在储存和发芽过程中对鼠诺如病毒、图兰病毒和甲型肝炎病毒的存活情况。
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