Waldbieser G C, Bosworth B G, Nonneman D J, Wolters W R
U.S. Department of Agriculture-Agricultural Research Service, Catfish Genetics Research Unit, Stoneville, Mississippi 38776, USA.
Genetics. 2001 Jun;158(2):727-34. doi: 10.1093/genetics/158.2.727.
Microsatellite loci were identified in channel catfish gene sequences or random clones from a small insert genomic DNA library. Outbred populations of channel catfish contained an average of eight alleles per locus and an average heterozygosity of 0.70. A genetic linkage map of the channel catfish genome (N = 29) was constructed from two reference families. A total of 293 microsatellite loci were polymorphic in one or both families, with an average of 171 informative meioses per locus. Nineteen type I loci, 243 type II loci, and one EST were placed in 32 multipoint linkage groups covering 1958 cM. Nine more type II loci were contained in three two-point linkage groups covering 24.5 cM. Twenty-two type II loci remained unlinked. Multipoint linkage groups ranged in size from 11.9 to 110.5 cM with an average intermarker distance of 8.7 cM. Seven microsatellite loci were closely linked with the sex-determining locus. The microsatellite loci and genetic linkage map will increase the efficiency of selective breeding programs for channel catfish.
微卫星位点是在斑点叉尾鮰基因序列或来自小插入片段基因组DNA文库的随机克隆中鉴定出来的。斑点叉尾鮰的远交群体每个位点平均含有8个等位基因，平均杂合度为0.70。利用两个参考家系构建了斑点叉尾鮰基因组的遗传连锁图谱（N = 29）。共有293个微卫星位点在一个或两个家系中表现出多态性，每个位点平均有171次信息性减数分裂。19个I型位点、243个II型位点和1个EST被定位到32个多点连锁群中，覆盖1958厘摩。另外9个II型位点包含在3个两点连锁群中，覆盖24.5厘摩。22个II型位点仍未连锁。多点连锁群的大小从11.9到110.5厘摩不等，标记间平均距离为8.7厘摩。7个微卫星位点与性别决定位点紧密连锁。这些微卫星位点和遗传连锁图谱将提高斑点叉尾鮰选择性育种计划的效率。
