Hoh B P, Siraj S S, Tan S G, Yusoff K
Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Jalan Hospital, Sg Buloh, Selangor, Malaysia.
Genet Mol Res. 2013 Feb 28;12(3):2578-93. doi: 10.4238/2013.February.28.1.
The river catfish Mystus nemurus is an important fresh water species for aquaculture in Malaysia. We report the first genetic linkage map of M. nemurus based on segregation analysis and a linkage map using newly developed microsatellite markers of M. nemurus. A total of 70 of the newly developed polymorphic DNA microsatellite markers were analyzed on pedigrees generated using a pseudo-testcross strategy from 2 mapping families. In the first mapping family, 100 offspring were produced from randomly selected dams of the same populations; dams of the second family were selected from 2 different populations, and this family had 50 offspring. Thirty-one of the 70 markers segregated according to the Mendelian segregation ratio. Linkage analysis revealed that 17 microsatellite markers belonging to 7 linkage groups were obtained at a logarithm of the odds score of 1.2 spanning 584 cM by the Kosambi mapping function, whereas the other 14 remained unlinked. The results from this study will act as primer to a more extensive genetic mapping study aimed towards identifying genetic loci involved in determining economically important traits.
河鲶鱼（Mystus nemurus）是马来西亚水产养殖中一种重要的淡水物种。我们报告了基于分离分析的河鲶鱼首张遗传连锁图谱，以及使用新开发的河鲶鱼微卫星标记构建的连锁图谱。在由2个作图家系采用拟测交策略产生的家系中，对总共70个新开发的多态性DNA微卫星标记进行了分析。在第一个作图家系中，从同一群体中随机选择的雌鱼产生了100个后代；第二个家系的雌鱼从2个不同群体中选择，该家系有50个后代。70个标记中有31个按照孟德尔分离比例进行分离。连锁分析表明，通过Kosambi作图函数，在对数优势分数为1.2时获得了属于7个连锁群的17个微卫星标记，跨度为584厘摩，而其他14个标记仍未连锁。本研究结果将为更广泛的遗传图谱研究提供基础，该研究旨在鉴定参与决定经济重要性状的基因座。
