de Veer M J, Holko M, Frevel M, Walker E, Der S, Paranjape J M, Silverman R H, Williams B R
Department of Cancer Biology, Lerner Research Institute, Cleveland, Ohio, USA.
J Leukoc Biol. 2001 Jun;69(6):912-20.
Interferons (IFNs) are a family of multifunctional cytokines that activate transcription of subsets of genes. The gene products induced by IFNs are responsible for IFN antiviral, antiproliferative, and immunomodulatory properties. To obtain a more comprehensive list and a better understanding of the genes regulated by IFNs, we compiled data from many experiments, using two different microarray formats. The combined data sets identified >300 IFN-stimulated genes (ISGs). To provide new insight into IFN-induced cellular phenotypes, we assigned these ISGs to functional categories. The data are accessible on the World Wide Web at http://www.lerner.ccf.org/labs/williams/, including functional categories and individual genes listed in a searchable database. The entries are linked to GenBank and Unigene sequence information and other resources. The goal is to eventually compile a comprehensive list of all ISGs. Recognition of the functions of the ISGs and their specific roles in the biological effects of IFNs is leading to a greater appreciation of the many facets of these intriguing and essential cytokines. This review focuses on the functions of the ISGs identified by analyzing the microarray data and focuses particularly on new insights into the protein kinase RNA-regulated (PRKR) protein, which have been made possible with the availability of PRKR-null mice.
干扰素（IFNs）是一类多功能细胞因子家族，可激活基因亚群的转录。IFNs诱导产生的基因产物具有IFN的抗病毒、抗增殖和免疫调节特性。为了获得更全面的受IFNs调控基因列表并更好地理解这些基因，我们使用两种不同的微阵列形式整合了多个实验的数据。合并后的数据集确定了超过300个干扰素刺激基因（ISGs）。为了深入了解IFN诱导的细胞表型，我们将这些ISGs归入功能类别。这些数据可在万维网http://www.lerner.ccf.org/labs/williams/上获取，包括功能类别以及可搜索数据库中列出的单个基因。条目与GenBank和Unigene序列信息及其他资源相关联。目标是最终编制一份所有ISGs的综合列表。对ISGs功能及其在IFNs生物学效应中的特定作用的认识，使人们对这些有趣且重要的细胞因子的多个方面有了更深入的了解。本综述重点关注通过分析微阵列数据确定的ISGs的功能，尤其关注对蛋白激酶RNA调节（PRKR）蛋白的新见解，这得益于PRKR基因敲除小鼠的可得性。
