Petin V G, Kim J K, Rassokhina A V, Zhurakovskaya G P
Biophysical Laboratory, Medical Radiological Research Center, 249030 Obninsk, Kaluga Region, Russia.
Mutat Res. 2001 Jul 1;478(1-2):169-76. doi: 10.1016/s0027-5107(01)00143-9.
In experiments with wild-type diploid yeast cells of Saccharomyces cerevisiae, the synergistic interaction of ultraviolet (UV) light (wavelength, 254 nm) and heat (45--60 degrees C) was studied both for mutagenic and inactivation effects. Simultaneous hyperthermia and UV light treatments increase the frequency of UV-induced mitotic intergenic recombination (crossing-over) and cell inactivation. The enhancing effect was a function of UV light fluence rate. It is concluded that the effect of hyperthermia on low fluence UV or high fluence UV irradiation results in comparable effects on survival and mitotic recombination suggesting similar modulation by hyperthermia of the effects induced by UV at different fluence rates. The interpretation of the data obtained was carried out within the widely accepted point of view considering the synergistic effects as a result of repair ability damage.
在对酿酒酵母野生型二倍体细胞进行的实验中，研究了紫外线（UV，波长254 nm）与热（45 - 60摄氏度）对诱变和失活效应的协同相互作用。同时进行热疗和紫外线处理会增加紫外线诱导的有丝分裂基因间重组（交叉互换）的频率以及细胞失活率。增强效应是紫外线通量率的函数。得出的结论是，热疗对低通量紫外线或高通量紫外线照射的影响，在存活和有丝分裂重组方面产生了可比的效应，这表明热疗对不同通量率紫外线诱导的效应具有相似的调节作用。所获得数据的解释是在广泛接受的观点范围内进行的，即将协同效应视为修复能力受损的结果。
