Machida I, Saeki T, Nakai S
Mutat Res. 1986 Mar;160(1):11-7. doi: 10.1016/s0027-5107(96)90003-2.
The effects of far (254 nm) and near (290-350 nm) ultraviolet (UV) light on mutations, intragenic and intergenic recombinations were compared in diploid strains of Saccharomyces cerevisiae. At equivalent survival levels there was not much difference in the induction of nonsense and missense mutations between far- and near-UV radiations. However, frameshift mutations were induced more frequently by near-UV than by far-UV radiation. Near-UV radiation induced intragenic recombination (gene conversion) as efficiently as far-UV radiation and the induced levels were similar in both radiations at equitoxic doses. A strikingly higher frequency was observed for the intergenic recombination induced by near-UV radiation than by far-UV radiation when compared at equivalent survival levels. Photoreactivation reduced the frequency only slightly in far-UV induced intergenic recombination and not at all in near-UV induction. These results indicate that near-UV damage involves strand breakage in addition to pyrimidine dimers and other lesions induced, whereas far-UV damage consists largely of photoreactivable lesions, pyrimidine dimers, and near-UV induced damage is more efficient for the induction of crossing-over.
在酿酒酵母的二倍体菌株中，比较了远紫外线（254纳米）和近紫外线（290 - 350纳米）对突变、基因内和基因间重组的影响。在同等存活水平下，远紫外线和近紫外线辐射在无义突变和错义突变的诱导方面没有太大差异。然而，近紫外线比远紫外线辐射更频繁地诱导移码突变。近紫外线辐射诱导基因内重组（基因转换）的效率与远紫外线辐射相同，并且在同等毒性剂量下，两种辐射的诱导水平相似。在同等存活水平下比较时，观察到近紫外线辐射诱导的基因间重组频率明显高于远紫外线辐射。光复活作用仅略微降低了远紫外线诱导的基因间重组频率，而对近紫外线诱导的基因间重组频率则完全没有影响。这些结果表明，近紫外线损伤除了嘧啶二聚体和其他诱导损伤外，还涉及链断裂，而远紫外线损伤主要由可光复活的损伤、嘧啶二聚体组成，并且近紫外线诱导的损伤在诱导交换方面更有效。
