Petin V G, Zhurakovskaya G P, Komarova L N
Biophysical Laboratory, Medical Radiological Research Center, Obninsk, Russia.
J Photochem Photobiol B. 1997 Apr;38(2-3):123-8. doi: 10.1016/s1011-1344(96)07449-0.
In experiments with wild-type diploid yeast cells of Saccharomyces cerevisiae, the synergistic lethal action of a simultaneous application of ultraviolet (UV) light (wavelength, 254 nm) and mild heat (45-57.5 degrees C) was studied. It was shown that, at any fixed UV light intensity, the synergistic effect occurred within the given temperature interval. The optimal temperature to achieve the greatest synergistic effect may be shown for every fluence rate examined. The correlation between the optimal temperature that maximized the synergy and UV light intensity was estimated: this temperature shifted towards higher temperature values with an increasing fluence rate. A possible interpretation of this effect is based on the supposition that the mechanism of the synergistic effect is related to additional lethal damage produced by the interaction of sublesions induced by each agent. These sublesions are supposed to be non-lethal when each agent is applied separately.
在对酿酒酵母野生型二倍体细胞进行的实验中，研究了同时施加紫外线（UV，波长254 nm）和温和加热（45 - 57.5摄氏度）的协同致死作用。结果表明，在任何固定的紫外线强度下，协同效应在给定的温度区间内出现。对于所检测的每个通量率，都可以显示出实现最大协同效应的最佳温度。估算了使协同作用最大化的最佳温度与紫外线强度之间的相关性：随着通量率的增加，该温度向更高温度值偏移。对这种效应的一种可能解释基于这样的假设，即协同效应的机制与每种试剂诱导的亚损伤相互作用产生的额外致死损伤有关。当分别应用每种试剂时，这些亚损伤被认为是非致死性的。
