[临床、动物及环境来源气单胞菌属中β-内酰胺类抗生素敏感性]

[Beta-lactam antibiotic sensitivity in Aeromonas spp. of clinical, animal, and environmental origin].

作者信息

Benassi F O, Vergara M, von Specht M H, García M A, Quiroga M I, Pucciarelli A B, Zubreski E, Laczeski M, Martin B M, Leardini N, Gutkind G

机构信息

Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Félix de Azara 1552, 3300 Posadas, Pcia. de Misiones.

出版信息

Rev Argent Microbiol. 2001 Jan-Mar;33(1):47-51.

PMID:11407020

Abstract

Susceptibility to beta-lactam antibiotics was investigated in Aeromonas spp. Microorganisms were isolated from both, clinical and water creek samples, as well as from processed raw chicken carcasses. Aeromonas like colonies were identified by means of Aerokey II and API 20 E System (Bio-Merieux). A. hydrophila prevailed both of human origin (44%) and water creek samples (41%), while A. caviae ranked first among raw chicken samples (65%). Dilution testing by Agar Method was performed to determine minimum inhibitory concentration (MIC), following NCCLS standards. All tested microorganisms were susceptible to third generation cephalosporin, cefepime, imipenem, aztreonam, and resistant to ampicillin. Only with cefepime and aztreonam exceptions, strains of human origin showed higher values of MIC90 than environmental ones. These results suggest that antibiotic resistance is mainly due to a steady environmental pressure, on account of the widely used above mentioned compounds.

摘要

对气单胞菌属细菌对β-内酰胺类抗生素的敏感性进行了研究。微生物从临床样本、溪水样本以及加工过的生鸡肉胴体中分离得到。利用Aerokey II和API 20 E系统（生物梅里埃公司）对气单胞菌样菌落进行鉴定。嗜水气单胞菌在人类来源样本（44%）和溪水样本（41%）中均占主导地位，而豚鼠气单胞菌在生鸡肉样本中占比最高（65%）。按照美国国家临床实验室标准委员会（NCCLS）标准，采用琼脂法进行稀释试验以确定最低抑菌浓度（MIC）。所有测试微生物对第三代头孢菌素、头孢吡肟、亚胺培南、氨曲南敏感，对氨苄西林耐药。除头孢吡肟和氨曲南外，人类来源的菌株MIC90值高于环境来源的菌株。这些结果表明，由于上述化合物的广泛使用，抗生素耐药性主要是由于持续的环境压力所致。