Srinivas M, Degaonkar M, Gupta D K, Raghunathan P, Das S N, Mitra D K
Department of Pediatric Surgery, All India Institute of Medical Sciences, New Delhi, 110029, India.
Pediatr Surg Int. 2001 May;17(4):316-20. doi: 10.1007/s003830100602.
For the assessment of germ-cell maturation of the seminiferous tubules, DNA flowcytometry is a rapid and sensitive method. Phosporus 31P magnetic resonance spectroscopy (MRS) is a non-invasive alternative that demonstrates the metabolic status of the testis, reflecting the type and relative proportion of germ cells in the testis. This study was designed to evaluate the utility of 31P MRS in reflecting the haploid-cell population of the testis as measured by DNA flowcytometry. A single testicle was evaluated in male Wistar pre-pubertal rats at 30, 40, 50, and 60 days of age. In order to minimize the contamination of signals from the contralateral testis, scrotum, and tail, the technique was modified and the testis was evaluated ex-vivo with an intact blood supply. At 30 days of age the percentage of haploid cells was 43.6 +/- 1.8, and this increased to 72.7 +/- 1.4 at 60 days of age. During this period, the testicular phosphomonoester/adenosine triphosphate (PM/ATP) ratio changed from 1.70 +/- 0.21 to 0.32 +/- 0.08. There was a significant (P < 0.001) linear correlation between the proportion of haploid cells evaluated by DNA flowcytometry and the PM/ATP ratio evaluated by 31P MRS. 31P MRS is thus a reliable, noninvasive technique for accurately assessing the status of the seminiferous epithelium.
对于评估生精小管中的生殖细胞成熟度，DNA流式细胞术是一种快速且灵敏的方法。磷31P磁共振波谱（MRS）是一种非侵入性的替代方法，可显示睾丸的代谢状态，反映睾丸中生殖细胞的类型和相对比例。本研究旨在评估31P MRS在反映通过DNA流式细胞术测量的睾丸单倍体细胞群体方面的效用。对30、40、50和60日龄的雄性Wistar青春期前大鼠的单个睾丸进行评估。为了尽量减少来自对侧睾丸、阴囊和尾巴信号的污染，对该技术进行了改进，并在保持完整血液供应的情况下对离体睾丸进行评估。30日龄时单倍体细胞的百分比为43.6±1.8，60日龄时增加到72.7±1.4。在此期间，睾丸磷酸单酯/三磷酸腺苷（PM/ATP）比值从1.70±0.21变为0.32±0.08。通过DNA流式细胞术评估的单倍体细胞比例与通过31P MRS评估的PM/ATP比值之间存在显著的（P<0.001）线性相关性。因此，31P MRS是一种可靠的非侵入性技术，可准确评估生精上皮的状态。
